{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T07:06:59Z","timestamp":1791356819106,"version":"4.3.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":189,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["RS-2023-00262645"],"award-info":[{"award-number":["RS-2023-00262645"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Trichosanthis Radix<\/jats:italic>\n                    (TR) is a pharmacopeial crude drug traditionally used to clear heat, reduce swelling, expel pus, and generate fluids, suggesting potential relevance to inflammatory skin disorders such as atopic dermatitis (AD). In this study, we evaluated the AD\u2010related activity of a TR water extract using an integrated workflow combining network\u2010based target analysis, marker compound analysis, and experimental validation in vivo and in vitro. The overlap between predicted TR targets and AD\u2010associated genes was analyzed using a protein\u2013protein interaction network, and extract was analyzed by high performance liquid chromatography (HPLC)\u2013evaporative light scattering detector (ELSD). Biological effects were examined in DNCB\u2010induced murine dermatitis and TNF\u2010\u03b1\/IFN\u2010\u03b3\u2010stimulated HaCaT keratinocytes. Twenty\u2010five overlapping genes formed a compact interaction module enriched for immune signaling and lipid\/nuclear receptor\u2010related pathways, and HPLC\u2013ELSD detected and quantified L\u2010citrulline as a marker compound for TR extract standardization. Topical TR reduced dermatitis severity, attenuated epidermal hyperplasia, and decreased dermal mast cell accumulation, with more consistent effects at the higher dose. TR also lowered lesional chemokine transcripts, particularly CCL17\/TARC and CCL5\/RANTES, and was associated with reduced NLRP3 and IL\u20101\u03b2. In addition, TR suppressed DNCB\u2010induced phosphorylation of MEK\/ERK and JAK2\u2013STAT1 and decreased p\u2010STAT1 immunofluorescence. In HaCaT cells, TR attenuated cytokine\u2010induced inflammatory effectors and phosphorylation of MEK\/ERK and STAT1. These findings indicate that TR water extract mitigates AD\u2010relevant inflammatory signaling while improving a barrier\u2010associated outcome, supporting further constituent\u2010resolved and mechanism\u2010directed studies.\n                  <\/jats:p>","DOI":"10.1155\/mi\/8744885","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:52:58Z","timestamp":1783597978000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Topical\n                    <i>Trichosanthis Radix<\/i>\n                    Water Extract Attenuates Atopic Dermatitis\u2010Like Skin Inflammation: Marker Standardization, Network Pharmacology, and Preclinical Validation"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0261-3537","authenticated-orcid":false,"given":"Hye-Min","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9022-5478","authenticated-orcid":false,"given":"Geun-Lip","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1018-8529","authenticated-orcid":false,"given":"Tae-Young","family":"Gil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha-Yeon","family":"Sim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5015-5949","authenticated-orcid":false,"given":"Hwan","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9234-7567","authenticated-orcid":false,"given":"Dong-Sung","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9893-5506","authenticated-orcid":false,"given":"Kyou-Young","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2937-874X","authenticated-orcid":false,"given":"Hyo-Jin","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.17544"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(25)00003-7"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/bjd\/ljad339"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2017.02.002"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-023-00992-4"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2018.10.3.207"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.4172\/2155-9899.1000110"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2022.2026920"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/bjd\/ljae342"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2016.10.005"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2023.09.001"},{"key":"e_1_2_13_12_2","volume-title":"Acute 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