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However, a functional understanding of these variants in immune cells central to disease development remains limited. We investigated oxylipin and cytokine production in resting and lipopolysaccharide (LPS)\u2010stimulated CD14+ monocytes from individuals carrying promoter variants and common alleles of\n                    <jats:italic>ALOX5<\/jats:italic>\n                    . Compared to monocytes from subjects carrying two common five\u2010repeat alleles (55 genotype), resting monocytes from individuals carrying one or more\n                    <jats:italic>ALOX5<\/jats:italic>\n                    deletion (d) alleles (d5 and dd genotypes) produced increased levels of IL\u20101\u03b2, IL\u20106, TNF\u2010\u0251, and IL\u201010 but lower quantities of putative trihydroxyeicosapenataenoic acid (TriHEPE) isomers detected with the same mass transition as resolvin E1 (RvE1) but slightly longer retention times. With the common 55 genotype, TriHEPEs increased as IL\u20106, IL\u201010, and TNF\u2010\u0251 production increased. This positive relationship between TriHEPEs and cytokines was diminished in monocytes with truncated\n                    <jats:italic>ALOX5<\/jats:italic>\n                    alleles. In response to LPS, monocytes from individuals with d5 and dd genotypes produced higher levels of IL\u20101\u03b2, IL\u20106, and TNF\u2010\u0251 but not IL\u201010, as well as increased quantities of cyclooxygenase (COX) products 11\u2010hydroxyeicosatetraenoic acid (11\u2010HETE), prostaglandin E2 (PGE\n                    <jats:sub>2<\/jats:sub>\n                    ), thromboxane B\n                    <jats:sub>2<\/jats:sub>\n                    (TxB\n                    <jats:sub>2<\/jats:sub>\n                    ), prostaglandin F2\u03b1 (PGF\n                    <jats:sub>2\u03b1<\/jats:sub>\n                    ), prostaglandin E1 (PGE\n                    <jats:sub>1<\/jats:sub>\n                    ), and prostaglandin B2 (PGB\n                    <jats:sub>2<\/jats:sub>\n                    ) compared to monocytes from individuals with the common 55 genotype. The observed changes in monocyte inflammatory mediator production provide a plausible link for the association of\n                    <jats:italic>ALOX5<\/jats:italic>\n                    deletion alleles and CVD risk.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Trial Registration:<\/jats:bold>\n                    Clinical trial: registered on ClinicalTrials.gov (Identifier: NCT00536185)\n                  <\/jats:p>","DOI":"10.1155\/mi\/8899139","type":"journal-article","created":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T06:33:57Z","timestamp":1778913237000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytokine and Oxylipin Production in Resting and LPS\u2010Stimulated Monocytes From Americans of African Ancestry Are Influenced by\n                    <i>ALOX5<\/i>\n                    Promoter Tandem Repeat Polymorphisms"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9896-5297","authenticated-orcid":false,"given":"Ryan G.","family":"Snodgrass","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles B.","family":"Stephensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hooman","family":"Allayee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel F.","family":"Snodgrass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin D.","family":"Laugero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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