{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:50:01Z","timestamp":1787017801512,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":105,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministerstwo Edukacji i Nauki","doi-asserted-by":"publisher","award":["S154\/2017"],"award-info":[{"award-number":["S154\/2017"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    The aim of the study was to find associations between the levels of liver injury serum markers and the selected liver, peripheral leukocytes, and plasma immune characteristics in biliary atresia (BA) children. Twenty\u2010five newly diagnosed BA children aged 4\u201330 weeks and 12 age\u2010matched controls were included (for leukocytes characteristics) and 19 BA children and 11 controls (for liver studies). The frequencies of T helper 1 (Th1), Th2, Th17, Th17.1 cells as well as numbers of regulatory T (Treg), B cell subsets, and matrix metalloproteinase \u22122 and \u22129 (MMP\u20102 and MMP\u20109) expressing leukocytes in the whole blood were evaluated by flow cytometry. Plasma concentrations of tissue inhibitors of metalloproteinase (TIMP)\u22121, \u22122, MMP\u20109, interleukin\u201017A (IL\u201017A) and IL\u20106 were assessed by enzyme\u2010linked immunosorbent assay (ELISA). The leukocyte and liver expression of the retinoic acid receptor\u2010related orphan nuclear receptor gamma (\n                    <jats:italic>ROR\u03b3T<\/jats:italic>\n                    ), fork\u2010head winged helix transcription factor P3 (\n                    <jats:italic>FoxP3<\/jats:italic>\n                    ), transforming growth factor beta (\n                    <jats:italic>TGF-\u03b2<\/jats:italic>\n                    ), interleukin\u201017A (\n                    <jats:italic>IL-17A<\/jats:italic>\n                    ),\n                    <jats:italic>IL-6<\/jats:italic>\n                    ,\n                    <jats:italic>IL-1\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic>IL-21<\/jats:italic>\n                    , interleukin 1 receptor antagonist (\n                    <jats:italic>IL-1Ra)<\/jats:italic>\n                    ,\n                    <jats:italic>MMP-2<\/jats:italic>\n                    ,\n                    <jats:italic>MMP-9<\/jats:italic>\n                    ,\n                    <jats:italic>MMP-12<\/jats:italic>\n                    (liver only),\n                    <jats:italic>TIMP-1<\/jats:italic>\n                    ,\n                    <jats:italic>TIMP-2<\/jats:italic>\n                    , T\u2010box transcription factor expressed in T cells, also called TBX21 (\n                    <jats:italic>T-bet<\/jats:italic>\n                    ), GATA\u2010binding protein 3 (\n                    <jats:italic>GATA3<\/jats:italic>\n                    ), and C\u2010type lectin (\n                    <jats:italic>CD161<\/jats:italic>\n                    ) mRNA were determined by real time RT\u2010PCR (reverse\u2010transcription polymerase chain reaction). The BA patients were characterized by increased frequencies of peripheral \u201csuppressor\u201d glycoprotein\u2010A repetitions predominant protein (GARP)\n                    <jats:sup>+<\/jats:sup>\n                    latency\u2010associated peptide (LAP)\n                    <jats:sup>+<\/jats:sup>\n                    Treg and activated Treg cells as well as MMP\u20102 and MMP\u20109 bearing lymphocytes, elevated plasma TIMP\u20101 levels, increased leukocyte expression of\n                    <jats:italic>MMP-9<\/jats:italic>\n                    ,\n                    <jats:italic>TIMP-1<\/jats:italic>\n                    ,\n                    <jats:italic>TIMP-2<\/jats:italic>\n                    ,\n                    <jats:italic>IL-6<\/jats:italic>\n                    , and\n                    <jats:italic>TGF-\u03b2<\/jats:italic>\n                    , and decreased leukocyte expression of IL\u201021 and T\u2010bet, increased liver expression of\n                    <jats:italic>FoxP3<\/jats:italic>\n                    ,\n                    <jats:italic>TIMP-1<\/jats:italic>\n                    , and decreased liver expression of\n                    <jats:italic>IL-1\u03b2<\/jats:italic>\n                    and\n                    <jats:italic>MMP-2<\/jats:italic>\n                    . The following correlations were found between serum markers of liver injury and leukocyte and liver immune characteristics: (a) hemoglobin (Hb) levels correlated negatively with frequency of peripheral \u201csuppressor\u201d GARP\n                    <jats:sup>+<\/jats:sup>\n                    LAP\n                    <jats:sup>+<\/jats:sup>\n                    Tregs; (b) aspartate aminotransferase (AST) levels correlated positively with frequency of the peripheral Th17.1 subset and expression of leukocyte\n                    <jats:italic>FoxP3<\/jats:italic>\n                    , (c) gamma glutamyltransferase (GGT) levels correlated positively with the peripheral memory B cells frequencies, the leukocyte\n                    <jats:italic>IL-6<\/jats:italic>\n                    and\n                    <jats:italic>TIMP-1<\/jats:italic>\n                    gene expression, (d) alanine aminotransferase (ALT) serum levels correlated positively with the na\u00efve B cell frequency and liver\n                    <jats:italic>TIMP-2<\/jats:italic>\n                    expression, (e) total bilirubin (Bil) levels correlated positively with the leukocyte\n                    <jats:italic>MMP-9<\/jats:italic>\n                    , the plasma IL\u20106 levels, and the liver\n                    <jats:italic>TIMP-2<\/jats:italic>\n                    gene expression, (f) direct Bil levels positively correlated with the liver\n                    <jats:italic>IL-6<\/jats:italic>\n                    and\n                    <jats:italic>TIMP-2<\/jats:italic>\n                    expression, (g) international normalized ratio of prothrombin time (PT\/INR) concentrations correlated positively with the peripheral Th17.1 subset frequency and the leukocyte\n                    <jats:italic>MMP-9<\/jats:italic>\n                    but negatively with the liver\n                    <jats:italic>FoxP3<\/jats:italic>\n                    expression. There were numerous strong positive correlations between the BA liver genes known to be involved in upregulation of IL\u201017 axis and MMPs\/TIMPs expression. No prevailing leukocyte or liver single markers were uniquely associated with serum liver injury indices. BA immune profile is very complex with no single characteristics that would distinguish it from other liver inflammatory diseases.\n                  <\/jats:p>","DOI":"10.1155\/mi\/9889239","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T04:19:47Z","timestamp":1744777187000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Correlation of the Expression Profile of Peripheral Leukocyte and Liver Tissue Immune Markers With Serum Liver Injury Indices in Children With Biliary 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