{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T10:44:15Z","timestamp":1790246655379,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>S100A8\/A9 (calprotectin), which is released by neutrophils under\ninflammatory conditions, has the capacity to induce apoptosis in\nvarious cells. We previously reported that S100A8\/A9 induces\napoptosis of EL\u20104 lymphoma cells via the uptake of extracellular\nzinc in a manner similar to DTPA, a membrane\u2010impermeable zinc\nchelator. In this study, S100A8\/A9\u2010induced apoptosis was examined\nin several cell lines that are weakly sensitive to DTPA,\nsuggesting S100A8\/A9 is directly responsible for apoptosis in\nthese cells. Since zinc inhibits apoptosis of MM46, one of these\ncells, the regulation by zinc of the capacity of S100A8\/A9 to bind\nMM46 cells was studied. When MM46 cells were incubated with\nS100A8\/A9 in standard or zinc\u2010depleted medium, the amounts of\nS100A8\/A9 bound to cells was markedly lower at 3 h than at\n1 h. In contrast, when MM46 cells were incubated with\nS100A8\/A9 in the presence of high levels of zinc, binding to cells\nwas the same at 1 and 3 h. When the cells were permeabilized\nwith saponin prior to analysis, a larger amount of cell\u2010associated\nS100A8\/A9 was detected at 3 h. The amount was further\nincreased in cells treated with chloroquine, suggesting that\nS100A8\/A9 was internalized and degraded in lysosomes. Although it\nhas been reported that S100A8\/A9 binds to heparan sulfate on cell\nmembranes, the amount of S100A8\/A9 bound to MM46 cells was not\nreduced by heparinase treatment, but was reduced by trypsin\ntreatment. These results suggest that S100A8\/A9 induces apoptosis by\ndirect binding to MM46 cells, and that this activity is regulated\nby zinc.<\/jats:p>","DOI":"10.1155\/mi.2005.280","type":"journal-article","created":{"date-parts":[[2005,10,24]],"date-time":"2005-10-24T12:15:36Z","timestamp":1130156136000},"page":"280-292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Regulation of S100A8\/A9 (Calprotectin) Binding to Tumor Cells byZinc Ion and Its Implication for Apoptosis\u2010Inducing Activity"],"prefix":"10.1155","volume":"2005","author":[{"given":"Yuichi","family":"Nakatani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatoshi","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter J.","family":"Chazin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Yui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,10,24]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2005\/763153.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/MI.2005.280","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:59:15Z","timestamp":1723075155000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/MI.2005.280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1155\/MI.2005.280"],"URL":"https:\/\/doi.org\/10.1155\/mi.2005.280","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1]]},"assertion":[{"value":"2005-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-06-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-10-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}