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This review summarizes current experimental and clinical evidence on the neurobiological processes associated with Tuina therapy, with a focus on pain modulation and neural repair. Available evidence suggests that mechanical stimulation during Tuina may engage mechanosensitive ion channels, including transient receptor\u2010potential vanilloid (TRPV)1, transient receptor\u2010potential ankyrin 1 (TRPA1), and Piezo channels, thereby modulating nociceptive signaling, nitric oxide (NO)\u2010cyclic guanosine monophosphate (cGMP)\u2010protein kinase G (PKG) and toll\u2010like receptor 4 (TLR4)\/nuclear factor kappa B (NF\u2010\u03baB)\u2010related inflammatory pathways, Piezo1\/Piezo2\u2010 and YAP\/TAZ\u2010associated mechanotransduction, neurotransmitters and neuropeptides, and descending pain inhibitory circuits involving the periaqueductal gray (PAG)\u2013rostral ventromedial medulla (RVM) system. In addition, emerging evidence indicates that Tuina may affect peripheral and central neuroplastic processes, including modulation of neuronal excitability, glial activity, and functional brain networks. While these findings provide a mechanistic framework for understanding reported clinical effects in conditions such as neuropathic pain (NP), low back pain (LBP), cervical disorders, and headache, heterogeneity in study design and intervention protocols limits definitive conclusions. Further well\u2010designed mechanistic studies and standardized clinical trials are required to clarify the role of Tuina in pain regulation and neuroplasticity and to support its evidence\u2010based application in clinical practice.<\/jats:p>","DOI":"10.1155\/np\/1624832","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T08:34:38Z","timestamp":1781512478000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mechanosensitive Ion Channels and Neuroplasticity in Tuina Therapy: Molecular Mechanisms and Therapeutic Implications"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0617-9035","authenticated-orcid":false,"given":"Mohammad","family":"Nasb","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2252-6989","authenticated-orcid":false,"given":"Dan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6020-2461","authenticated-orcid":false,"given":"Dandan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8336-7278","authenticated-orcid":false,"given":"Jing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5181-0367","authenticated-orcid":false,"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-02903-w"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0321771"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.62347\/QAUW2551"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.2196\/52820"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v31.i44.113017"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11726-019-1098-7"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/jebm.12522"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2024.1399487"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1155\/prm\/3697374"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctim.2024.103098"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2025.1705466"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-025-05794-0"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.1096734"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-025-01105-1"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38241-x"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jbmr\/zjae074"},{"key":"e_1_2_14_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/2437396"},{"key":"e_1_2_14_18_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6652099"},{"key":"e_1_2_14_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/5859298"},{"key":"e_1_2_14_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2023.997116"},{"key":"e_1_2_14_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000033018"},{"key":"e_1_2_14_22_2","doi-asserted-by":"publisher","DOI":"10.1089\/acm.2017.0209"},{"key":"e_1_2_14_23_2","doi-asserted-by":"publisher","DOI":"10.3390\/healthcare9030257"},{"key":"e_1_2_14_24_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000001382"},{"key":"e_1_2_14_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.1007432"},{"key":"e_1_2_14_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2006.02.006"},{"key":"e_1_2_14_27_2","volume-title":"Applied Neurophysiology","author":"Simpson J. 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