{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:26:27Z","timestamp":1789683987487,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":73,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neural Plasticity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>Various forms of mild stress may exacerbate pain in patients with chronic pain disorders, though the underlying mechanism remains unclear. Astrocyte activation in the spinal dorsal horn plays a predominant role in stress and pain. The present study investigated the neuron\u2013astrocyte interactions in the spinal dorsal horn in post\u2010traumatic stress disorder (PTSD)\u2010induced hyperalgesia using a single\u2010prolonged stress (SPS) model, a Complete Freund\u2019s Adjuvant (CFA) model and an SPS\u2009+\u2009CFA model. Animals were tested for mechanical withdrawal threshold (MWT) of the paw after SPS, CFA and SPS\u2009+\u2009CFA. SPS\u2009+\u2009CFA group induced significantly increased mechanical allodynia compared with the SPS or CFA group. We tested the hypothesis that IL\u20101\u03b2 contributes to signaling between astrocytes and neurons in stress\u2010induced hyperalgesia (SIH). Immunohistochemical data showed that there was an upregulation of glial fibrillary acidic proteins (GFAPs, a marker of astrocyte) and Fos (a marker of neuron) in SIH. Immunohistochemical data showed specific localization of IL\u20101\u03b2 to astrocyte, but not to microglia and neurons and a neuronal localization of the IL\u20101\u03b2 receptor (IL\u20101RI) with NMDAR2B (NR2B). Enzyme immunoassay analysis showed that IL\u20101\u03b2 release was dependent on c\u2010Jun N\u2010terminal kinase (JNK) activation in astrocyte. The JNK inhibitor SP600125 suppressed IL\u20101\u03b2 release. SP600125 and IL\u20101RI blockade with IL\u20101ra resulted in a restoration of behavioral nociceptive thresholds. Our results showed that the IL\u20101\u03b2\u2010dependent, JNK\u2010regulated astrocyte\u2013neuron signaling pathway mediated the astroglia component of pain maintenance in SIH.<\/jats:p>","DOI":"10.1155\/np\/2791699","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T03:55:20Z","timestamp":1773633320000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A JNK\u2010Regulated and IL\u20101\u03b2\u2010Dependent Astrocyte\u2013Neuron Signaling Pathway in the Spinal Dorsal Horn is Essential for Stress\u2010Induced Hyperalgesia"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6413-8795","authenticated-orcid":false,"given":"Jian","family":"Qi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,15]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2024.110067"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep39442"},{"key":"e_1_2_10_3_2","first-page":"251","volume-title":"Current Topics in Behavioral Neurosciences","author":"Olango W. 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