{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T06:10:02Z","timestamp":1723097402202},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2191,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2191,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"American Paralysis Association"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neural Plasticity"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p>The effects of implantation of cultured\nadrenal medullary cells on the recovery of\nneurotransmitter specific reflex activity were\nstudied in the rat spinal cord using electrophysiological\ntesting methods. Cell suspensions\nof cultured neonatal adrenal medullary\nchromaffin (AM) cells (which produce\ncatecholamines), or Schwann (Sc) cells (controls)\nwere implanted into the lumbar region of the\nspinal cord 2 weeks after catecholamine (CA)\ndenervation by intracisternal injection of 6\u2010hydroxydopamine\n(6\u2010OHDA). All cells were\ntaken from 7 day neonates and cultured for 10\ndays in the presence of nerve growth factor\n(NGF). Three months after implantation, the\nextent of implant\u2010associated recovery of reflex\nactivity was determined by measuring electromyogram\n(EMG) activity and force associated\nwith the long latency component of the hindlimb\nwithdrawal reflex (which is CA modulated).\nAfter the electrophysiological testing, rats were\nanesthetized, and the spinal cords were rapidly\nremoved and frozen. Spinal cords were sectioned\nlongitudinally, and implanted cells were\nvisualized using glyoxylic acid techniques.\nLabelled sections were examined to determine\ncell survival. Results indicate that 1) chromaffin\ncells survive for 3 months in the segments of the\ncord into which they have been implanted and\n2) rats implanted with AM cells have\nsignificantly more forceful withdrawal reflexes\nthan those that received Sc cells or received no\nimplant after lesioning.<\/jats:p>","DOI":"10.1155\/np.1994.89","type":"journal-article","created":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T09:22:50Z","timestamp":1173950570000},"page":"89-102","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Cultured Adrenal Chromaffin Cell Implants on Hindlimb Reflexes of the 6\u2010OHDA Lesioned Rat"],"prefix":"10.1155","volume":"5","author":[{"given":"Bruce E.","family":"Pulford","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea R.","family":"Mihajlov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard O.","family":"Nornes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Ray","family":"Whalen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,1]]},"container-title":["Neural Plasticity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/np\/1994\/106848.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/NP.1994.89","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T05:45:18Z","timestamp":1723095918000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/NP.1994.89"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1155\/NP.1994.89"],"URL":"https:\/\/doi.org\/10.1155\/np.1994.89","archive":["Portico"],"relation":{},"ISSN":["2090-5904","1687-5443"],"issn-type":[{"type":"print","value":"2090-5904"},{"type":"electronic","value":"1687-5443"}],"subject":[],"published":{"date-parts":[[1994,1]]},"assertion":[{"value":"2000-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}