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However, due to synergistic interactions of polyherbal ingredients, the positive effects of MA mixed with CT are still controversial. Preliminary outcomes from in vitro assessment exposed that a 3:1 ratio of MA:CT (MACT) yielded the highest antioxidant capacity and gamma\u2010aminobutyric acid (GABA)\u2010promoting activity among seven combination ratios: 0MA:1CT, 1MA:0CT, 1MA:1CT, 1MA:2CT, 1MA:3CT, 2MA:1CT, and 3MA:1CT. Male Wistar rats (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096\/group) were electroencephalographically and electromyographically monitored to confirm the sedative\u2010hypnotic function of the assigned ingredients over 3\u2009h after oral administration. Cognitive and anxiolytic effects were also evaluated thereafter. Following drug administration, it was found that MACT exhibited positive influences in a dose\u2010dependent manner (125, 250, and 500\u2009mg\/kg), and in a significantly better manner than either 500\u2009mg\/kg MA or CT alone. Interestingly, a majority of these effects, including sedative\u2010hypnotic parameters, that is, decreasing of latency to rapid eye movements (REMs) sleep and wake duration, increasing of REM sleep duration, number of REM sleep bouts, as well as elevating of cognitive function and anxiolytic parameters was reversed by pretreatment with bicuculline methiodide (2\u2009mg\/kg), a GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor antagonist. Overall, the advantages of MACT\u2010based polyherbal drugs, which act preferentially on GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptors, may pave the way for further development of MACT as an alternative drug or food supplement for neuropharmacological improvements in GABA\n                    <jats:sub>A<\/jats:sub>\n                    receptor\u2010related disorders.\n                  <\/jats:p>","DOI":"10.1155\/omcl\/2305848","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T20:51:38Z","timestamp":1771102298000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Functional Ingredient Derived From a Mixture of Mulberry (\n                    <i>Morus alba<\/i>\n                    L.) Leaves and Butterfly Pea (\n                    <i>Clitoria ternatea<\/i>\n                    L.) 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