{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:41:50Z","timestamp":1791236510163,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":348,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parkinson's Disease"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Oxidative stress is a major contributor to the pathogenesis of Parkinson\u2019s disease, promoting neuronal degeneration through the production of excessive reactive oxygen species. In this context, natural products such as essential oils are attracting increasing attention for their potential to protect neurons. Clove essential oil (CEO), which is extracted from the flower buds of the plant Syzygium aromaticum, is renowned for its antioxidant activity. This study aimed to investigate the chemical composition, antioxidant properties, and protective effects of CEO on SH\u2010SY5Y cells, which are used as a cellular model of Parkinson\u2019s disease. The CEO was obtained by hydrodistillation (yield: 15%) and is primarily composed of eugenol (84.49%) and acetyl\u2010eugenol (10.05%). Its antioxidant activity was confirmed via DPPH radical scavenging (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20090.081\u2009\u00b1\u20090.001\u2009mg\/mL) and iron chelation (110.32\u2009\u00b1\u20090.67\u2009mg EDTA equivalent\/g essential oil (EO)). qRT\u2010PCR, Western blot, and slot blot techniques demonstrated that CEO pretreatment at concentrations of 2.5, 5, and 10\u2009\u03bcg\/mL significantly reduced 6\u2010OHDA\u2010induced oxidative DNA damage and restored the gene and protein expression of key antioxidant enzymes (GPx1, GPx4, SOD1, and CAT). These results highlight the powerful antioxidant and neuroprotective properties of CEO, supporting its potential as a therapeutic agent for neurodegenerative disorders related to oxidative stress, such as Parkinson\u2019s disease.\n                  <\/jats:p>","DOI":"10.1155\/padi\/4243787","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:33:58Z","timestamp":1765805638000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clove Essential Oil Enhances Antioxidant Defenses and Reduces DNA Damage in a Cellular Model of Parkinson\u2019s Disease"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5687-8337","authenticated-orcid":false,"given":"Dhouha","family":"Hamdi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Ouachikh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayhan","family":"Kocer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lemlih","family":"Ouchchane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chokri","family":"Messaoud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9833-8485","authenticated-orcid":false,"given":"Aziz","family":"Hafidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,15]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1051\/medsci\/2011274405"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-017-0340-9"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.14-42"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-024-03696-4"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0033291708003681"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2019.5347"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1769"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112236"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-89437-4"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants11111476"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13425"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201000088"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0023-6438(95)80008-5"},{"key":"e_1_2_13_14_2","first-page":"9","article-title":"Antioxidant Properties of Guava Fruits: Comparison with Some Local Fruits","volume":"3","author":"Yan L. 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