{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:45:02Z","timestamp":1792118702103,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":239,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pulmonary Medicine"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Endobronchial ultrasound\u2010guided transbronchial needle aspiration (EBUS\u2010TBNA) is an established procedure for diagnosing thoracic diseases and staging of lung cancers. However, some limitations of cytology specimens from EBUS\u2010TBNA include small sample size, low tumour cellularity, necrosis and specimen contamination. Endobronchial ultrasound\u2010guided transbronchial mediastinal cryobiopsy (EBUS\u2010TBMC) is a promising alternative that provides a larger histology specimen which may improve diagnostic accuracy and molecular testing. This study is aimed at evaluating the benefits of EBUS\u2010TBMC over EBUS\u2010TBNA, focusing on improving next\u2010generation sequencing (NGS) success rates, and assessing its efficacy and safety in a real\u2010world setting.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    Data from 203 patients (99 underwent EBUS\u2010TBNA and 104 underwent EBUS\u2010TBMC) were retrospectively traced and analysed using descriptive statistics.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The overall diagnostic yield was significantly higher for EBUS\u2010TBMC (90.38%) than that for EBUS\u2010TBNA (67.68%;\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001). For heterogeneous lesions, the diagnostic yield was 92.31% for EBUS\u2010TBMC and 69.44% for EBUS\u2010TBNA (\n                    <jats:italic>p<\/jats:italic>\n                    = 0.011). For non\u2010small\u2010cell lung cancer (NSCLC), EBUS\u2010TBMC specimens demonstrated higher overall tumour cellularity (65% vs. 30%;\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001) and better success in detecting driver alterations through NGS (85.36% vs. 61.90%;\n                    <jats:italic>p<\/jats:italic>\n                    = 0.035). The median procedure duration was shorter for EBUS\u2010TBMC (22 vs. 32\u2009min;\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.001), and the complication rates were comparable between the two techniques. These findings suggest that EBUS\u2010TBMC offers additional diagnostic advantages over EBUS\u2010TBNA for heterogeneous lesions and significantly facilitates the acquisition of cell\u2010rich specimens for NGS testing.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    EBUS\u2010TBMC increases the overall diagnostic yield of mediastinal diseases. EBUS\u2010TBMC provides cell\u2010rich histology specimens with high tumour content, facilitating NGS testing in the management of NSCLC.\n                  <\/jats:p>","DOI":"10.1155\/pm\/3522554","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T12:35:17Z","timestamp":1756384517000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Performance of Endobronchial Ultrasound\u2010Guided Cryobiopsy in Diagnosing Thoracic Disorders and Its Role in Next\u2010Generation Sequencing for Non\u2010Small\u2010Cell Lung Cancer"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8560-7888","authenticated-orcid":false,"given":"Chun Ian","family":"Soo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1457-9231","authenticated-orcid":false,"given":"Sze Shyang","family":"Kho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6418-8888","authenticated-orcid":false,"given":"Wai Ling","family":"Leong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinye","family":"Eng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5067-3217","authenticated-orcid":false,"given":"Diana Bee-Lan","family":"Ong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seow Fan","family":"Chiew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tak Kuan","family":"Chow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazwan Amzar Khairul","family":"Annuar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chee Kuan","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong Kin","family":"Liam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.12-2355"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00097908"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.15-1216"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2013.05.066"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.21037\/med-20-28"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00055-2021"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1183\/23120541.00448-2022"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(22)00392-7"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.3348\/jksr.2015.72.4.271"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-2006"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00006412"},{"key":"e_1_2_12_12_2","article-title":"Technical Tips, Diagnostic Yield and Safety of Endobronchial Ultrasound-Guided Transbronchial Mediastinal Cryobiopsy","volume":"79","author":"Soo C. 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