{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:13:48Z","timestamp":1783426428318,"version":"3.54.6"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":187,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pulmonary Medicine"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Multidrug\u2010resistant tuberculosis (MDR\u2010TB) is becoming a major threat to the global control of tuberculosis (TB). The situation worsens when fluoroquinolone (FQ) and second\u2010line injectable (SLID) resistant strains are involved. This study assesses the utility of MTBDR\n                      <jats:italic>sl<\/jats:italic>\n                      V2.0 line probe assay (LPA) in identifying resistance patterns to FQ and SLID in MDR\u2010TB isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 74 MDR\u2010TB isolates were tested for genetic mutations that confer resistance to FQs and against SLIDs by applying the GenoType MTBDR\n                      <jats:italic>sl<\/jats:italic>\n                      V2.0 LPA. This assay targets resistance\u2010associated mutations in the\n                      <jats:italic>gyrA<\/jats:italic>\n                      and\n                      <jats:italic>gyrB<\/jats:italic>\n                      genes for FQ resistance, and in the\n                      <jats:italic>rrs<\/jats:italic>\n                      and\n                      <jats:italic>eis<\/jats:italic>\n                      genes for resistance to aminoglycosides and capreomycin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 74 isolates examined, 14 (18.9%) had FQ resistance. Most of these isolates had mutations in the\n                      <jats:italic>gyrA<\/jats:italic>\n                      gene, most commonly at codon A90V (6.7%), followed by D94G (5.4%), and various D94 variations. No mutations were found in the rrs, gyrB, or eis genes, and no resistance to SLIDs was found. With no discernible contribution from\n                      <jats:italic>rrs, gyrB<\/jats:italic>\n                      , or\n                      <jats:italic>eis<\/jats:italic>\n                      genes, our results emphasize\n                      <jats:italic>gyrA<\/jats:italic>\n                      mutations, particularly A90V and D94G, as important molecular markers of FQ resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The MTBDR\n                      <jats:italic>sl<\/jats:italic>\n                      V2.0 assay demonstrated operational utility for rapid detection of resistance\u2010associated mutations related to FQs and SLID drugs in MDR\u2010TB isolates. Its rapid turnaround time expanded mutation coverage, and applicability in routine diagnostic settings make it a useful tool for molecular resistance profiling in high TB\u2010burden settings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/pm\/6993694","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T11:17:21Z","timestamp":1783423041000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Detection of Resistance Mutations in Multidrug\u2010Resistant Tuberculosis With GenoType MTBDR\n                    <i>sl<\/i>\n                    Assay"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1010-0224","authenticated-orcid":false,"given":"Shaina","family":"Gaikwad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7453-3023","authenticated-orcid":false,"given":"Antisha","family":"Tiwari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-6076","authenticated-orcid":false,"given":"Jitendra","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7490-3953","authenticated-orcid":false,"given":"Alkesh Kumar","family":"Khurana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8126-4459","authenticated-orcid":false,"given":"Sagar","family":"Khadanga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3542-2401","authenticated-orcid":false,"given":"Shashank","family":"Purwar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7557-3693","authenticated-orcid":false,"given":"Debasis","family":"Biswas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4827-919X","authenticated-orcid":false,"given":"Anand Kumar","family":"Maurya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"e_1_2_12_1_2","volume-title":"Global Tuberculosis Report 2022 [Internet]","author":"World Health Organization","year":"2022"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmyco.2012.08.001"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00081-09"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2022.115856"},{"key":"e_1_2_12_5_2","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.20546\/ijcmas.2019.810.118","article-title":"Detection of Pre-extensively Drug Resistance [PRE-XDR TB] and Extensively Drug Resistance [XDR-TB] Among Pulmonary Multidrug Resistant Tuberculosis [MDR-TB] Patient by Line Probe Assay","volume":"8","author":"Gadhavi H.","year":"2019","journal-title":"International Journal of Current Microbiology and Applied Sciences"},{"key":"e_1_2_12_6_2","unstructured":"LifeScience. H. GenoType MTBDRsl VER 1.0 Instructions for Use. Document IFU-317A-03. Nehren Germany: HAIN LifeScience. Document IFU-317A-03. HAIN LifeScience Nehren 2010."},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.04.049"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijmy.ijmy_135_18"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD015543"},{"key":"e_1_2_12_10_2","article-title":"Diagnostic Accuracy Operational Feasibility and Cost Considerations of Truenat MTB Plus and Xpert MTB\/RIF Ultra for Pulmonary Tuberculosis","author":"Sidiq Z.","year":"2026","journal-title":"medRxiv"},{"key":"e_1_2_12_11_2","doi-asserted-by":"crossref","first-page":"e175","DOI":"10.1016\/S2666-5247(20)30060-4","article-title":"Whole-Genome Sequencing of Mycobacterium tuberculosis directly from clinical samples for High-Resolution Genomic Epidemiology and Drug Resistance Surveillance: An Observational Study","volume":"1","author":"Goig G. 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