{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:25:43Z","timestamp":1787034343943,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":228,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pain Research and Management"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The analgesic effects of esketamine have been reported. MiR\u2010153\u20133p is a central player in multiple pathologies of neural injury. The potential interplay between them remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study investigated whether the analgesic effects of esketamine in neuropathic pain were mechanistically linked to miR\u2010153\u20133p.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Neuropathic pain was induced in rats through chronic constriction injury (CCI) of the sciatic nerve, followed by esketamine administration. Pain hypersensitivity was assessed by dynamic changes in paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL). MiR\u2010153\u20133p expression was manipulated via intrathecal injection of lentiviral\u2010delivered miR\u2010153\u20133p mimic. RT\u2010qPCR was employed for analysis of miR\u2010153\u20133p expression and AKT3 mRNA levels. Proinflammatory cytokine levels were quantified by ELISA, while protein expression was detected using Western blot. The interaction between miR\u2010153\u20133p and AKT3 was verified by RIP and luciferase reporter assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Esketamine alleviated CCI\u2010induced neuropathic pain by elevating PWMT and PWTL. Esketamine also reduced proinflammatory cytokine release. MiR\u2010153\u20133p was significantly reduced in CCI rats, and esketamine administration effectively reversed its decline. MiR\u2010153\u20133p upregulation suppressed pain hypersensitivity and proinflammatory cytokines. AKT3 was a target of miR\u2010153\u20133p. AKT3 knockdown abolished the effect of MiR\u2010153\u20133p inhibition on esketamine protection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Esketamine mitigated neuropathic pain and reduced proinflammatory cytokine release through the miR\u2010153\u20133p\/AKT3 pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/prm\/5536335","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:40:34Z","timestamp":1787031634000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Esketamine Alleviates Neuropathic Pain and Inflammation via miR\u2010153\u20133p\/AKT3 in CCI Rats"],"prefix":"10.1155","volume":"2026","author":[{"given":"Xiumei","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7182-1809","authenticated-orcid":false,"given":"Yawen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_10_1_2","article-title":"Gabapentin for Chronic Neuropathic Pain and Fibromyalgia in Adults","volume":"3","author":"Moore R. 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