{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T01:10:02Z","timestamp":1723079402578},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2191,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2191,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p>The effects of human tumour necrosis factor\u2010\u03b1 (TNF\u03b1), or its\nmutein (F4168) having the cell adhesive Arg\u2010Gly\u2010Asp sequence at the\nN\u2010terminus, on intestinal injury, were examined. Histopathological\nexamination revealed that an intravenous injection of TNF\u03b1\nresulted in marked haemorrhage or oedema in the caecum of rats,\nwhereas F4168 showed no such effects even at the same therapeutic\ndose. Moreover, the number of neutrophils that adhered to\nendothelial cells or infiltrated the mucosal tissue was much higher\nafter TNF\u03b1 injection compared with F4168 <jats:italic>in vivo<\/jats:italic>.\nThe enhanced adhesion of neutrophils on to human umbilical vein\nendothelial cells also occurred when the latter were pre\u2010stimulated\nwith TNF\u03b1 but not with F4168 <jats:italic>in vitro<\/jats:italic>. The\nexpression of the cell adhesion molecules including endothelial\nleukocyte adhesion molecule\u20101 or intercellular adhesion molecule\u20101\non F4168\u2010 stimulated human umbilical vein endothelial ceils was\nsignificantly lower than that stimulated with TNF\u03b1. These\nresults suggest that the Arg\u2010Gly\u2010Asp sequence introduced into the\nTNF\u03b1 molecule abrogates the side effect of this cytokine such\nas tissue injury or shock, and that F4168 could be useful for\nsystemic therapy.<\/jats:p>","DOI":"10.1155\/s096293519400013x","type":"journal-article","created":{"date-parts":[[2007,3,14]],"date-time":"2007-03-14T11:14:27Z","timestamp":1173870867000},"page":"111-116","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A novel Mutein of TNF\u03b1 Containing the Arg\u2010Gly\u2010Asp Sequence Shows Reduced Toxicity in Intestine"],"prefix":"10.1155","volume":"3","author":[{"given":"H.","family":"Shikama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Miyata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Sakae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Yotsuya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/1994\/357909.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/1994\/357909.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/S096293519400013X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T23:58:33Z","timestamp":1723075113000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/S096293519400013X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1155\/S096293519400013X"],"URL":"https:\/\/doi.org\/10.1155\/s096293519400013x","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"type":"print","value":"0962-9351"},{"type":"electronic","value":"1466-1861"}],"subject":[],"published":{"date-parts":[[1994,1]]},"assertion":[{"value":"2000-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}