{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:31:03Z","timestamp":1783348263671,"version":"3.54.6"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":1461,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004099","name":"Ligue Nationale Contre le Cancer","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:p>The production of nitric oxide (NO) was measured in cultures of spleen cells stimulated by lipopolysaccharide (LPS), IL\u20102 or LPS + IL\u20102. We observed that NO synthesis is increased by IFN\u2010\u03b3 but inhibited by IFN\u2010\u03b1\/\u03b2. This is not the case when IL\u20102 is present in the cultures, since interferons play a minor role in the regulation of the NO production. When IL\u20102 and LPS were associated in the cultures, the IFN\u2010\u03b1\/\u03b2 role seems more important than that of IFN\u2010\u03b3. PGE<jats:sub>2<\/jats:sub> inhibits NO production in LPS supplemented cultures but has a slight effect in the presence of IL\u20102 and no effect with IL\u20102 + LPS. 3\u2010isoButyl\u20101\u2010methylxanthine (IBMX), an inhibitor of phosphodiesterases, induces a decrease of IFN production. In the presence of H\u20107, an inhibitor of protein kinase C (PKC), NO production is reduced when the cultures are supplemented by LPS or IL\u20102 but not when IL\u20102 and LPS are both added. H\u20107 also reduced IFN production. In the presence of N<jats:sup>G<\/jats:sup>\u2010monomethyl\u2010L\u2010arginine (N\u2010MMA), an inhibitor of NO synthesis, IFN production was increased, with no change in the cytotoxic activity. Hence, interferons regulate NO production by mouse spleen cells and, in return, NO modulates the generation of IFN.<\/jats:p>","DOI":"10.1155\/s0962935196000117","type":"journal-article","created":{"date-parts":[[2007,3,14]],"date-time":"2007-03-14T11:16:45Z","timestamp":1173871005000},"page":"62-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nitric oxide production in murine spleen cells: role of interferons and prostaglandin E<sub>2<\/sub> in the generation of cytotoxic activity"],"prefix":"10.1155","volume":"5","author":[{"given":"Dominique","family":"Vaillier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Daculsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Gualdel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1996,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/1996\/351280.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/S0962935196000117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:40:12Z","timestamp":1723070412000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/S0962935196000117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,1]]}},"alternative-id":["10.1155\/S0962935196000117"],"URL":"https:\/\/doi.org\/10.1155\/s0962935196000117","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,1]]},"assertion":[{"value":"1996-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}