{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T13:49:23Z","timestamp":1760622563995},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":1461,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Glaxo Wellcome"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[1996,1]]},"abstract":"<jats:p>Hyperresponsiveness of the airways to nonspecific stimuli is a characteristic feature of asthma. Airway responsiveness is usually characterized in terms of the position and shape of the dose\u2013response curve to methacholine (MDR). In the study we have investigated the influence of fluticasone propionate (FP), a topically active glucocorticoid, on arachidonic acid (AA) metabolites in broncho\u2010alveolar lavage (BAL) fluid (i.e. TxB<jats:sub>2<\/jats:sub>, PGE<jats:sub>2<\/jats:sub>, PGD<jats:sub>2<\/jats:sub>, 6kPGF<jats:sub>1\u03b1<\/jats:sub> and LTC<jats:sub>4<\/jats:sub>) on the one hand and MDR curves on the other hand. The effect of FP was studied in a randomized, double\u2010blind, placebo\u2010controlled design in 33 stable nonsmoking asthmatics; 16 patients received FP (500 \u03bcg b.i.d.) whereas 17 patients were treated with placebo. We found that the forced expiratory volume in 1s (FEV<jats:sub>1<\/jats:sub> % predicted) increased, the log<jats:sub>2<\/jats:sub>PC<jats:sub>20<\/jats:sub> methacholine increased and the plateau value (% fall in FEV<jats:sub>1<\/jats:sub>) decreased after a 12 week treatment period. No changes in AA\u2010metabolites could be determined after treatment except for PGD<jats:sub>2<\/jats:sub> which decreased nearly significantly (p = 0.058) within the FP treated group, whereas the change of PGD<jats:sub>2<\/jats:sub> differed significantly (<jats:italic>p<\/jats:italic> = 0.05) in the FP treated group from placebo. The levels of the other AA metabolites (i.e. TxB<jats:sub>2<\/jats:sub>, PGE<jats:sub>2<\/jats:sub>, 6kPGF<jats:sub>1\u03b1<\/jats:sub> and LTC<jats:sub>4<\/jats:sub>) remained unchanged after treatment and were not significantly different from the placebo group. Our results support the hypothesis that although FP strongly influences the position, the shape and also the maximum response plateau of the MDR curve, this effect is not mainly achieved by influence on the level of AA metabolites. Other pro\u2010inflammatory factors may be of more importance for the shape of the MDR curve. It is suggested that these pro\u2010inflammatory factors are downregulated by FP.<\/jats:p>","DOI":"10.1155\/s0962935196000324","type":"journal-article","created":{"date-parts":[[2007,3,14]],"date-time":"2007-03-14T11:16:45Z","timestamp":1173871005000},"page":"224-229","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of fluticasone propionate on arachidonic acid metabolites in BAL\u2010fluid and methacholine dose\u2010response curves in non\u2010smoking atopic asthmatics"],"prefix":"10.1155","volume":"5","author":[{"given":"S. E.","family":"Overbeek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"Bogaard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. M.","family":"Garrelds","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. J.","family":"Zijlstra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. G. H.","family":"Mulder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. C.","family":"Hoogsteden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,1]]},"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/1996\/916392.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/S0962935196000324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T11:40:52Z","timestamp":1723117252000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/S0962935196000324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,1]]}},"alternative-id":["10.1155\/S0962935196000324"],"URL":"https:\/\/doi.org\/10.1155\/s0962935196000324","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"type":"print","value":"0962-9351"},{"type":"electronic","value":"1466-1861"}],"subject":[],"published":{"date-parts":[[1996,1]]},"assertion":[{"value":"1996-01-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}