{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T20:40:03Z","timestamp":1723063203730},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:p><jats:italic>Objective:<\/jats:italic> This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.<\/jats:p><jats:p><jats:italic>Methods:<\/jats:italic> Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT\u2010PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and \u03b2\u2010actin mRNA levels.<\/jats:p><jats:p><jats:italic>Results:<\/jats:italic> eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were \u2264 3.12 \u00d7 10<jats:sup>-4<\/jats:sup>, \u2264 3.12 \u00d7 10<jats:sup>-4<\/jats:sup>, 6.24 \u00d7 10<jats:sup>-4<\/jats:sup> and 2.5 \u00d7 10<jats:sup>-3<\/jats:sup> attomoles\/\u03bcl, respectively. \u03b2\u2010actin mRNA levels for all tissues were between 1.25 \u00d7 10<jats:sup>-1<\/jats:sup> and 6.25 \u00d7 10<jats:sup>-2<\/jats:sup> attomoles\/\u03bcl.<\/jats:p><jats:p><jats:italic>Conclusion:<\/jats:italic> eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.<\/jats:p>","DOI":"10.1155\/s1064744901000199","type":"journal-article","created":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T13:08:38Z","timestamp":1173272918000},"page":"113-116","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Adhesion Development and the Expression of Endothelial Nitric Oxide Synthase"],"prefix":"10.1155","volume":"9","author":[{"given":"David M.","family":"Svinarich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fadi M.","family":"Zaher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lena","family":"Holmdahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasser","family":"Chegini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Gonik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Diamond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,3,7]]},"container-title":["Infectious Diseases in Obstetrics and Gynecology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/idog\/2001\/284191.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/idog\/2001\/284191.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/S1064744901000199","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T19:52:34Z","timestamp":1723060354000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/S1064744901000199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1155\/S1064744901000199"],"URL":"https:\/\/doi.org\/10.1155\/s1064744901000199","archive":["Portico"],"relation":{},"ISSN":["1064-7449"],"issn-type":[{"type":"print","value":"1064-7449"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2000-11-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-03-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-03-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}