{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T18:53:39Z","timestamp":1762541619875},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p><jats:italic>Objective:<\/jats:italic> To identify alterations in the cytokine profile and microbial ecosystem of the vagina in association\nwith cervical dysplasia.<\/jats:p><jats:p><jats:italic>Methods:<\/jats:italic> Demographics, lifestyle variables and Papanicolau (Pap) smear results of subjects presenting to the\nsame site for gynecologic complaints, obstetric visits or colposcopy were prospectively recorded. Vaginal smear\nfor Gram stain, aerobic and anaerobic culture, pH, and wet mount and KOH examination for <jats:italic>Trichomonas vaginalis<\/jats:italic> ,\n<jats:italic>Gardnerella vaginalis<\/jats:italic> and yeast organisms were performed. Vaginal lavage specimens were centrifuged, and the\npellets and supernatants were assayed for human papillomavirus (HPV) by polymerase chain reaction and for\ncytokines interleukin (IL)\u20101\u00df IL\u20106, IL\u201010 and IL\u201012 by enzyme\u2010linked immunosorbent assay (ELISA) respectively.\nSubjects with abnormal Pap smears underwent colposcopy and biopsy as indicated.<\/jats:p><jats:p>Results: Of 51 patients, 32 were referred for colposcopy, 12 presented with gynecologic needs, and seven\npresented for obstetric visits. Median age was 24 years. Demographics did not differ significantly between the\ndysplasia and control groups except for a trend towards more sexual partners in the dysplasia group. Biopsies were\nperformed in 81% (26\/32) of patients presenting for colposcopy and 17 revealed cervical intraepithelial neoplasia.\nIL\u20101\u00df, IL\u20106, IL\u201010, and IL\u201012 levels were elevated in 63% (20\/32), 38% (15\/39), 4% (2\/49), and 0% of samples\nrespectively. Elevated vaginal lavage IL\u20101\u00df was associated with a 6.1 odds ratio (95% confidence interval 1.06\u201335)\nof cervical dysplasia. Alterations in other variables studied were not associated with cervical dysplasia.<\/jats:p><jats:p>Conclusions: Elevated IL\u20101\u00df, possibly representing a complex host inflammatory response to multiple pathogens,\nwas demonstrated in patients with cervical dysplasia.<\/jats:p>","DOI":"10.1155\/s1064744902000200","type":"journal-article","created":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T13:12:46Z","timestamp":1173273166000},"page":"181-186","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Role of the Vaginal Microbiological Ecosystem and Cytokine Profile in the Promotion of Cervical Dysplasia: A Case\u2013Control Study"],"prefix":"10.1155","volume":"10","author":[{"given":"Kian","family":"Behbakht","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Friedman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ira","family":"Heimler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alla","family":"Aroutcheva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose","family":"Simoes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Faro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,6,14]]},"container-title":["Infectious Diseases in Obstetrics and Gynecology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/idog\/2002\/658314.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/S1064744902000200","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T21:23:09Z","timestamp":1723065789000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/S1064744902000200"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1155\/S1064744902000200"],"URL":"https:\/\/doi.org\/10.1155\/s1064744902000200","archive":["Portico"],"relation":{},"ISSN":["1064-7449"],"issn-type":[{"type":"print","value":"1064-7449"}],"subject":[],"published":{"date-parts":[[2002,1]]},"assertion":[{"value":"2002-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-06-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-06-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}