{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T17:46:47Z","timestamp":1757699207112},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2191,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":2191,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Eleanor Naylor Dana Charitable Trust"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p><jats:italic>Objective:<\/jats:italic><jats:italic>Trichomonas vaginalis<\/jats:italic> vaginal infections are often both asymptomatic and difficult to\ndetect by current methods. We evaluated the ability of a newly developed polymerase chain reaction (PCR) assay to\nidentify <jats:italic>T. vaginalis<\/jats:italic> in vaginal samples from pregnant and non\u2010pregnant women.<\/jats:p><jats:p><jats:italic>Methods:<\/jats:italic> In the 1st study, we compared the prevalence of <jats:italic>T. vaginalis<\/jats:italic> detection by PCR and culture\nusing Diamond\u2032s medium in 52 women with symptoms of vaginal infection. In the 2nd study, <jats:italic>T. vaginalis<\/jats:italic> was detected \nusing PCR and wet mount microscopy in 131 asymptomatic pregnant women.<\/jats:p><jats:p><jats:italic>Results:<\/jats:italic> Among the women with symptoms of vaginitis, 7 (13.5%) were PCR\u2010positive for <jats:italic>T. vaginalis<\/jats:italic>.\nSix of the PCR\u2010positive women, but none of the PCR\u2010negative women, were culture\u2010positive for this organism. All but 1 of the\nwomen with candidal vaginitis or bacterial vaginosis were PCR\u2010negative for T. <jats:italic>vaginalis<\/jats:italic>. Among the asymptomatic pregnant \nwomen, all of whom were negative for <jats:italic>T. vaginalis<\/jats:italic> by wet mount, l0 (7.6%) were PCR\u2010positive for <jats:italic>T. vaginalis<\/jats:italic>.<\/jats:p><jats:p><jats:italic>Conclusions:<\/jats:italic> PCR offers a rapid and sensitive alternative to culture and microscopy for the detection\nof <jats:italic>T. vaginalis<\/jats:italic> vaginal infections in both symptomatic and asymptomatic women.<\/jats:p>","DOI":"10.1155\/s1064744994000335","type":"journal-article","created":{"date-parts":[[2007,3,7]],"date-time":"2007-03-07T13:01:22Z","timestamp":1173272482000},"page":"16-19","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Detection of <i>Trichomonas vaginalis<\/i> Using the Polymerase Chain Reaction in Pregnant andNon\u2010Pregnant Women"],"prefix":"10.1155","volume":"2","author":[{"given":"J.","family":"Jeremias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Draper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ziegert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Inglis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"McGregor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. S.","family":"Witkin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,5,24]]},"container-title":["Infectious Diseases in Obstetrics and Gynecology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/idog\/1994\/718703.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/S1064744994000335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T21:37:30Z","timestamp":1723066650000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/S1064744994000335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1155\/S1064744994000335"],"URL":"https:\/\/doi.org\/10.1155\/s1064744994000335","archive":["Portico"],"relation":{},"ISSN":["1064-7449"],"issn-type":[{"type":"print","value":"1064-7449"}],"subject":[],"published":{"date-parts":[[1994,1]]},"assertion":[{"value":"1993-12-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1994-05-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}