{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:06:20Z","timestamp":1789211180739,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":254,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"\n Preeclampsia (PE) is a pregnancy\u2010related disorder associated with significant maternal and fetal morbidity, and intrauterine conditions may influence the biological behavior of human umbilical cord (UC)\u2010derived mesenchymal stem cells (hUC\u2010MSCs). However, comparative data between early\u2010onset PE (EOPE) and late\u2010onset PE (LOPE) remain limited. This study aimed to compare the in vitro growth kinetics of hUC\u2010MSCs derived from pregnancies complicated by EOPE and LOPE. In this comparative in vitro study, UC samples (\n n<\/jats:italic>\n \u2009=\u200966) were classified into three groups according to the maternal clinical condition: control (\n n<\/jats:italic>\n \u2009=\u200922), EOPE (\n n<\/jats:italic>\n \u2009=\u200922), and LOPE (\n n<\/jats:italic>\n \u2009=\u200922). Cells were isolated using a mechanical explant method and cultured under standard conditions. Culture parameters, including cell\u2010outgrowth time, time to 90% confluency (\n P<\/jats:italic>\n 0<\/jats:sub>\n ), first passage duration (\n P<\/jats:italic>\n 1<\/jats:sub>\n ), and total culture duration (\n P<\/jats:italic>\n 0<\/jats:sub>\n +\n P<\/jats:italic>\n 1<\/jats:sub>\n ), were recorded. Population doubling time (PDT) was calculated using both outgrowth\u2010adjusted and\n P<\/jats:italic>\n 1<\/jats:sub>\n \u2010based approaches, and phenotypic characterization was performed by flow cytometry. Cell\u2010outgrowth time did not differ significantly among the groups (\n p<\/jats:italic>\n = 0.930).\n P<\/jats:italic>\n 0<\/jats:sub>\n duration differed significantly among the groups (\n p<\/jats:italic>\n < 0.001). EOPE\u2010derived cells exhibited a shorter\n P<\/jats:italic>\n 0<\/jats:sub>\n duration (19.00\u2009\u00b1\u20092.19 days) than both control\u2010derived cells (21.65\u2009\u00b1\u20091.63 days; Tukey\u2010adjusted\n p<\/jats:italic>\n = 0.0004) and LOPE\u2010derived cells (20.71\u2009\u00b1\u20092.35 days; Tukey\u2010adjusted\n p<\/jats:italic>\n = 0.0266).\n P<\/jats:italic>\n 1<\/jats:sub>\n duration and total culture duration did not differ significantly among the groups (\n p<\/jats:italic>\n = 1.000 and\n p<\/jats:italic>\n = 0.090, respectively). The outgrowth\u2010adjusted group\u2010level PDT estimate was lower in EOPE (16.6\u2009h) than in LOPE (18.4\u2009h) and controls (19.1\u2009h), whereas the\n P<\/jats:italic>\n 1<\/jats:sub>\n \u2010based PDT estimates were closely similar across the groups (~32\u2009h). Total cell yield and viability were numerically similar across the groups. These findings indicate that EOPE\u2010derived hUC\u2010MSCs exhibit an early culture\u2010specific kinetic difference, predominantly reflected by a shorter\n P<\/jats:italic>\n 0<\/jats:sub>\n duration, without a sustained difference in subsequent\n P<\/jats:italic>\n 1<\/jats:sub>\n expansion or total culture duration. The accelerated early growth may reflect adaptive responses to the intrauterine environment rather than enhanced functional potential, suggesting that early culture parameters may serve as sensitive indicators of disease\u2010related alterations.\n <\/jats:p>","DOI":"10.1155\/sci\/3980640","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:24:31Z","timestamp":1789208671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Early\u2010Onset Preeclampsia\u2010Derived hUC\u2010MSCs Exhibit Accelerated Initial Culture Kinetics Without Sustained Growth Advantage: A Comparative In Vitro Study"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0622-6687","authenticated-orcid":false,"given":"\u0130brahim","family":"Alptekin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5061-2775","authenticated-orcid":false,"given":"Bilge","family":"Alptekin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2703-0138","authenticated-orcid":false,"given":"Ezel","family":"Erkan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8254-0558","authenticated-orcid":false,"given":"Ferda Topal","family":"Celikkan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1866-7295","authenticated-orcid":false,"given":"Derya Uyan","family":"Hendem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8567-9048","authenticated-orcid":false,"given":"Dilek","family":"\u015eahin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5393-6115","authenticated-orcid":false,"given":"Alp","family":"Can","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,12]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.31143"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2016.03.001"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms262211091"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2019.07.004"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2023.104172"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0013"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13679-024-00599-4"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25094869"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm13092668"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2025.07.077"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002213"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.111.00250"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.01.021"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.05.054"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24166"},{"key":"e_1_2_13_16_2","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1016\/j.placenta.2008.11.003","article-title":"Placental Endoplasmic Reticulum Stress and Oxidative Stress in the Pathophysiology of Unexplained Intrauterine Growth Restriction and Early Onset Preeclampsia","author":"Burton G. J.","year":"2009","journal-title":"Placenta 30"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.923334"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/428726"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127992"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0439"}],"container-title":["Stem Cells International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci\/3980640","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/sci\/3980640","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci\/3980640","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:24:33Z","timestamp":1789208673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/sci\/3980640"}},"subtitle":[],"editor":[{"given":"Satabdi","family":"Datta Choudhury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/sci\/3980640"],"URL":"https:\/\/doi.org\/10.1155\/sci\/3980640","archive":["Portico"],"relation":{},"ISSN":["1687-966X","1687-9678"],"issn-type":[{"value":"1687-966X","type":"print"},{"value":"1687-9678","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3980640"}}