{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:05:35Z","timestamp":1790751935958,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":271,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82200759"],"award-info":[{"award-number":["82200759"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Stem Cells International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Neurogenic bladder (NB) is characterized by chronic inflammation and bladder tissue injury, and current treatments fail to restore the inflammatory microenvironment or tissue integrity. Although adipose\u2010derived stem cell (ADSC) therapy shows regenerative potential, its efficacy is limited by inflammation; whether CD73 overexpression enhances ADSC therapeutic effects in NB remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A rat model of NB was established via pelvic nerve (PN) injury and treated with CD73\u2010overexpressing ADSCs (CD73\n                      <jats:sup>+<\/jats:sup>\n                      ADSCs). Bladder function, histological remodeling, and apoptosis were evaluated in vivo. In vitro, conditioned medium (CM) from CD73\n                      <jats:sup>+<\/jats:sup>\n                      ADSCs was applied to LPS\u2010injured rat bladder transitional epithelial cells (RB\u2010TECs) and macrophages to assess proliferation, migration, apoptosis, inflammatory responses, and macrophage polarization. Key signaling pathways were examined, and label\u2010free quantitative (LFQ) proteomics was performed to characterize global molecular reprogramming.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CD73\n                      <jats:sup>+<\/jats:sup>\n                      ADSCs significantly improved urodynamic function, restored smooth muscle architecture, and reduced apoptosis in NB rats. In vitro, CD73\n                      <jats:sup>+<\/jats:sup>\n                      ADSC\u2010CM promoted urothelial cell proliferation and migration while attenuating cell death. Mechanistically, CD73\n                      <jats:sup>+<\/jats:sup>\n                      ADSCs enhanced extracellular adenosine production, at least partly dependent on pharmacologically sensitive A2A receptor signaling, suppressed NF\u2010\u03baB activation, reduced proinflammatory cytokine release, promoted macrophage polarization toward an M2 phenotype, and inhibited NLRP3 inflammasome activation. Proteomic analysis revealed a global shift toward antiinflammatory and reparative molecular programs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings suggest that CD73 contributes to the immunomodulatory and reparative properties of ADSCs under inflammatory conditions. By coordinating adenosinergic signaling, inflammatory suppression, and repair\u2010associated pathways, CD73\n                      <jats:sup>+<\/jats:sup>\n                      ADSCs effectively reprogram the NB microenvironment and represent a promising regenerative therapeutic strategy for NB.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/sci\/8564160","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:49:38Z","timestamp":1790750978000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CD73\u2010Overexpressing ADSCs Promote Neurogenic Bladder Repair via A2A\u2013JAK1\/STAT3\u2010Mediated Modulation of Inflammation and Macrophage 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