{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:37:17Z","timestamp":1772987837795,"version":"3.50.1"},"reference-count":69,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":335,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20252BAC240465"],"award-info":[{"award-number":["20252BAC240465"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009102","name":"Education Department of Jiangxi Province","doi-asserted-by":"publisher","award":["JXJG-23-13-27"],"award-info":[{"award-number":["JXJG-23-13-27"]}],"id":[{"id":"10.13039\/501100009102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010857","name":"Jiangxi Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["409126659006"],"award-info":[{"award-number":["409126659006"]}],"id":[{"id":"10.13039\/501100010857","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Gastrointestinal parasites, being members of the neglected tropical diseases (NTDs), infect over one billion individuals, about 24% of the global population. The aim of this study was to evaluate the deworming potential of\n                      <jats:italic>Piper nigrum, Albizia ferruginea<\/jats:italic>\n                      , and\n                      <jats:italic>Guarea cedrata<\/jats:italic>\n                      against\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                      and\n                      <jats:italic>Heligmosomoides polygyrus<\/jats:italic>\n                      and to recommend their use in traditional medicine for the treatment of helminth infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The anthelmintic properties of the extracts were investigated in two nematode strains,\n                      <jats:italic>Heligmosomoides polygyrus<\/jats:italic>\n                      and\n                      <jats:italic>Caenorhabditis elegans.<\/jats:italic>\n                      The fresh coprocultured\n                      <jats:italic>H. polygyrus<\/jats:italic>\n                      L3 larvae and\n                      <jats:italic>C. elegans<\/jats:italic>\n                      L4 larvae bleached from adult worms were used to investigate the properties. Larval movement was monitored using a worm microtracker in a 96\u2010well microplate to quantify the anthelmintic action of the extracts. The extracts were screened at varying concentrations, with distilled water being the negative control and albendazole being the positive control. Percent inhibition of larval motility was calculated. Molecular docking studies were also carried out using the Glide module of Schrodinger Maestro software, and the results ranked and distinguished based on the software\u2019s scoring function.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The most active extract against\n                      <jats:italic>H. polygyrus<\/jats:italic>\n                      was the ethanolic extract of\n                      <jats:italic>Piper nigrum<\/jats:italic>\n                      (IC\n                      <jats:sub>50<\/jats:sub>\n                      :0.04\u2009mg\/mL) followed by the aqueous extract of\n                      <jats:italic>Piper nigrum<\/jats:italic>\n                      (IC\n                      <jats:sub>50<\/jats:sub>\n                      :0.08\u2009mg\/mL). Aqueous and ethanoic extracts of\n                      <jats:italic>Piper nigrum<\/jats:italic>\n                      were active against\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                      L4 larvae with IC\n                      <jats:sub>50<\/jats:sub>\n                      s of 7.850 and 16.17\u2009\u00b5g\/mL, respectively, while aqueous extracts of\n                      <jats:italic>Guarea cedrata<\/jats:italic>\n                      and\n                      <jats:italic>Albizia ferruginea<\/jats:italic>\n                      were highly active with IC\n                      <jats:sub>50<\/jats:sub>\n                      s of 3.235 and 4.729\u2009\u03bcg\/mL, respectively. Leucokinin III, Leucokinin I, Leucokinin VIII, Leucokinin II, and Rebaudioside C from\n                      <jats:italic>Albizia ferruginea<\/jats:italic>\n                      are the most potent compounds against succinate dehydrogenase (SDH) and \u03b2\u2010tubulin. Each of these constituents exhibited a more pronounced effect compared to the positive control, albendazole. Tricholein, isopiperolein B, pipercyclobutanamide, piperettine, and piperine from\n                      <jats:italic>Piper nigrum<\/jats:italic>\n                      are the most potent compounds against SDH and \u03b2\u2010tubulin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study has demonstrated in vitro and in silico the effectiveness of\n                      <jats:italic>Piper nigrum, Albizia ferruginea,<\/jats:italic>\n                      and\n                      <jats:italic>Guarea cedrata<\/jats:italic>\n                      toward helminthiasis. To validate this scientific investigation, more research is required, particularly on the acute toxicity and in vivo anthelmintic efficacy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/sci5\/1600933","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T07:22:53Z","timestamp":1764746573000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anthelminthic Activity of\n                    <i>Piper nigrum<\/i>\n                    ,\n                    <i>Albizia ferruginea<\/i>\n                    , and\n                    <i>Guarea cedrata<\/i>\n                    Against\n                    <i>Caenorhabditis elegans<\/i>\n                    and\n                    <i>Heligmosomoides polygyrus<\/i>\n                    : In Vitro and In Silico Molecular Docking Approach"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2393-9841","authenticated-orcid":false,"given":"Noumedem Anangmo","family":"Christelle Nadia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7558-4296","authenticated-orcid":false,"given":"Yamssi","family":"C\u00e9dric","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Djam Chefor","family":"Alain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ndongmo Donjio","family":"Corine Leader","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3952-0073","authenticated-orcid":false,"given":"Masoud","family":"Besati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5511-9346","authenticated-orcid":false,"given":"Mahdi","family":"Yaghoobi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9526-7653","authenticated-orcid":false,"given":"Wenjuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5004-9011","authenticated-orcid":false,"given":"Ye","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6805-4142","authenticated-orcid":false,"given":"Haibo","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2010.06.009"},{"key":"e_1_2_11_2_2","unstructured":"World Health Organization (WHO) Assessing the Epidemiology of soil-transmitted Helminths During a Transmission Assessment Survey (TAS) Within the Framework of the Global Programme to Eliminate Lymphatic Filariasis 2015."},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001582"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.2147\/rmhp.s295974"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/1178633720943509"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.19044\/esj.2017.v13n33p276"},{"key":"e_1_2_11_7_2","unstructured":"Pautric-ThomasS. FonvieilleM. J.-L. Ma\u00eetreM. P. J. andDorchiesM. P. Recent Data on Anthelmintic Resistance of Gastrointestinal Strongyles in Ruminants 2003 National Veterinary School of Toulouse PhD Thesis."},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2004.07.010"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0921-4488(02)00047-0"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2023.07.004"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2010.1065"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6628787"},{"key":"e_1_2_11_13_2","first-page":"54","article-title":"Comparative Anthelmintic Activity and Phytochemical Evaluation of Tridax procumbens Linn Whole Plant Extract and Piper nigrum Linn Seed Extract on Indian Adult Earthworm","volume":"27","author":"Patil V.","year":"2014","journal-title":"International Journal of Pharmaceutical Sciences Review and Research"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.2024792"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s43088-022-00196-1"},{"key":"e_1_2_11_16_2","first-page":"2161","article-title":"Invivo Anti-trypanosomal Activity of the Leaf Extracts of Albizia schimperiana (Fabaceae) Against Trypanosoma Congolense Infection in Mice","volume":"5","author":"Tesfaye A.","year":"2015","journal-title":"Clinical and Experimental Pharmacology"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5359669"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6690869"},{"key":"e_1_2_11_19_2","first-page":"1","article-title":"Evaluation of Anthelmintic Activity of Lagenaria siceraria (Molina) Standl and Albizia lebbeck L. Against Gastrointestinal Helminths of Sheep","volume":"5","author":"Khan M.","year":"2010","journal-title":"Egyptian Journal of Sheep and Goats Sciences"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-4017(03)00218-8"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/s2221-1691(12)60524-3"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/10412905.1995.9698502"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.55705\/cmbr.2022.351720.1051"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.109877"},{"key":"e_1_2_11_25_2","unstructured":"MohammedG. OmranA. andHusseinH. Antibacterial and Phytochemical Analysis of Piper nigrum Using Gas Chromatography\u2013Mass Spectrum and 2016."},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s43094-022-00405-9"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s42269-022-00716-0"},{"key":"e_1_2_11_28_2","first-page":"1284","article-title":"GC-MS Analysis of Albizia lebbeck Benth","volume":"11","author":"Bobby M. N.","year":"2015","journal-title":"WJPR"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.microb.2024.100150"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27248758"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/6735764"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2023.11.053"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232415739"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15782-7"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2021.113246"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7332"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13555"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00299a024"},{"key":"e_1_2_11_39_2","volume-title":"Maestro","author":"Maestro S.","year":"2020"},{"key":"e_1_2_11_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm0306430"},{"key":"e_1_2_11_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/cimb44030068"},{"key":"e_1_2_11_42_2","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2020.1715258"},{"key":"e_1_2_11_43_2","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/8564163"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2013.06.035"},{"key":"e_1_2_11_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.commatsci.2018.03.042"},{"key":"e_1_2_11_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15085734"},{"key":"e_1_2_11_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-024-01650-w"},{"key":"e_1_2_11_48_2","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/1858154"},{"key":"e_1_2_11_49_2","doi-asserted-by":"publisher","DOI":"10.5897\/ajb12.2297"},{"key":"e_1_2_11_50_2","doi-asserted-by":"publisher","DOI":"10.1107\/s2053229621007841"},{"key":"e_1_2_11_51_2","article-title":"Caenorhabditis elegans: a Model to Study the Anthelmintic Effects of Polyphenolic Compounds on the Fertility of Parasitic Gastrointestinal Nematodes?","author":"Arroyo-Lopez C.","year":"2020","journal-title":"bioRxiv"},{"key":"e_1_2_11_52_2","doi-asserted-by":"publisher","DOI":"10.2478\/helm-2021-0004"},{"key":"e_1_2_11_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/med\/9780190933043.003.0007"},{"key":"e_1_2_11_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu15051206"},{"key":"e_1_2_11_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.06.006"},{"key":"e_1_2_11_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vas.2024.100395"},{"key":"e_1_2_11_57_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-11-106"},{"key":"e_1_2_11_58_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci6020035"},{"key":"e_1_2_11_59_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389557516666151120121036"},{"key":"e_1_2_11_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2018.12.008"},{"key":"e_1_2_11_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-23566-2"},{"key":"e_1_2_11_62_2","doi-asserted-by":"publisher","DOI":"10.20884\/1.jap.2017.19.1.595"},{"key":"e_1_2_11_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2021.109505"},{"key":"e_1_2_11_64_2","first-page":"945","article-title":"Comparative Phytochemistry and in Vitro Antimicrobial Effectiveness of the Leaf Extracts of Clerodendrum capitatum (Verbenaceae) and Ficus glumosa (Moraceae)","volume":"6","author":"Usman H.","year":"2017","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_11_65_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-023-03722-0"},{"key":"e_1_2_11_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2021.116458"},{"key":"e_1_2_11_67_2","doi-asserted-by":"publisher","DOI":"10.21608\/puj.2024.292672.1250"},{"key":"e_1_2_11_68_2","doi-asserted-by":"publisher","DOI":"10.3390\/insects13080676"},{"key":"e_1_2_11_69_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389203717999160226180733"}],"container-title":["Scientifica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/1600933","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/sci5\/1600933","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/1600933","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:08:10Z","timestamp":1772968090000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/sci5\/1600933"}},"subtitle":[],"editor":[{"given":"Vikram","family":"Dalal","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":69,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/sci5\/1600933"],"URL":"https:\/\/doi.org\/10.1155\/sci5\/1600933","archive":["Portico"],"relation":{},"ISSN":["2090-908X","2090-908X"],"issn-type":[{"value":"2090-908X","type":"print"},{"value":"2090-908X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2025-06-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1600933"}}