{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:06:14Z","timestamp":1790899574358,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Dendrobium lineale<\/jats:italic>\n                    Rolfe is an important orchid species used as a parent in breeding. Currently, this orchid is classified as an endangered species and is listed in Appendix II according to CITES. This study aimed to investigate the effects of sucrose and coconut water (CW) on the asymbiotic seed germination and shoot development of this endangered Indonesian orchid.\n                    <jats:italic>In vitro<\/jats:italic>\n                    orchid seed culture aids in conservation and reintroduction. Three\u2010month\u2010old hand\u2010pollinated seeds were sown on Vacin and Went (VW) solid medium supplemented with several concentrations of sucrose (10, 20, and 30\u2009g\/L) or without sucrose (controls). Seed germination and protocorm development were investigated 1, 2, and 3\u2009months after sowing. To analyze the role of CW in subsequent shoot development and root formation, shoots with an approximately 1\u2010cm length and comprising 1\u20102 leaves obtained following seed germination were cultured on VW medium supplemented with several concentrations of CW (5%, 10%, 15%, and 20%) and without CW (control) treatment. After 4\u2009months of culture, the number of roots, the number of leaves, and dry weight of the plantlets were recorded. VW medium containing 20\u2009g\/L sucrose effectively enhanced seed germination (89%) and shoot formation with one or more leaves (stage 5) (46%). Supplementation with 20% CW in VW medium was suitable for shoot development, resulting in well\u2010developed roots and leaves and improved fresh weight of plantlets compared with those in the other treatments. Moreover, 87% of the acclimatized plantlets survived. This protocol is an efficient method for the in vitro mass production and conservation of this endangered epiphytic orchid using asymbiotic seed culture.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/2153196","type":"journal-article","created":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T06:42:05Z","timestamp":1767422525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Improvement of In Vitro Seed Germination and Shoot Development of the Indonesian Endangered Orchid,\n                    <i>Dendrobium lineale<\/i>\n                    Rolfe, Using Sucrose and Coconut Water"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6635-9954","authenticated-orcid":false,"given":"Edy Setiti Wida","family":"Utami","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3924-0805","authenticated-orcid":false,"given":"Sri","family":"Lestari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1149-1103","authenticated-orcid":false,"given":"Dwi Kusuma","family":"Wahyuni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0151-222X","authenticated-orcid":false,"family":"Junairiah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0562-2058","authenticated-orcid":false,"given":"Hery","family":"Purnobasuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0712-9259","authenticated-orcid":false,"given":"Sucipto","family":"Hariyanto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabila","family":"Istighfari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7942-875X","authenticated-orcid":false,"given":"Ahmad Affan Ali","family":"Murtadlo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti","family":"Umamah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,2]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/boj.12234"},{"key":"e_1_2_10_2_2","volume-title":"State of the World\u2019s Plants 2017 Report","author":"Willis K. 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