{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:13:47Z","timestamp":1791987227335,"version":"4.4.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"vor","delay-in-days":117,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    This study aimed to investigate the phytochemical composition, antibacterial activity, and antitumor potential of\n                    <jats:italic>Hibiscus rosa-sinensis<\/jats:italic>\n                    Linn, a widely used medicinal plant. Qualitative systematic phytochemical analysis was conducted on flower of\n                    <jats:italic>Hibiscus rosa-sinensis<\/jats:italic>\n                    for determining their antimicrobial and antitumor potential by using disc diffusion method and potato disc bioassay, respectively. Phytochemicals found in chloroform extract of\n                    <jats:italic>H. rosa-sinensis<\/jats:italic>\n                    were alkaloids, betacyanin\u2019s, tannins, and quinones in nature while in ethanol extract only alkaloids and betacyanin\u2019s were present. The findings demonstrated potent inhibitory effects of the extracts against both Gram\u2010positive and Gram\u2010negative bacteria. Chloroform extract exhibited more significant antibacterial activity as compared to ethanolic extract against various drug resistant pathogens such as\n                    <jats:italic>Salmonella typhi<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli, Staphylococcus aureus, Klebsiella<\/jats:italic>\n                    sp.\n                    <jats:italic>, Pseudomonas aeruginosa<\/jats:italic>\n                    , and\n                    <jats:italic>Bacillus subtilis.<\/jats:italic>\n                    Furthermore, the antitumor activity of the\n                    <jats:italic>H. rosa-sinensis<\/jats:italic>\n                    extract was evaluated using in vitro assays against selected cancer cell\u2010lines. These extracts exhibited significant antiproliferative effects such as ethanolic extract had more significant antitumor activity as compared to chloroform. After 24\u2009h zones of inhibition were analyzed and measured using ruler. The diameter of zones of inhibition of chloroform flower extract against\n                    <jats:italic>E. coli, S. aureus, Klebsiella<\/jats:italic>\n                    sp.\n                    <jats:italic>, Pseudomonas aeruginosa<\/jats:italic>\n                    , and\n                    <jats:italic>B. subtilis, S. typhi<\/jats:italic>\n                    was 11, 8, 13, 17, 10, and 23\u2009mm, while ethanolic extract had 6, 0, 6, 10, 7, and 10\u2009mm, zone of inhibition, respectively. Flower extracts by inhibiting the formation of crown gall tumors suggesting their potential as sources of natural anticancer compounds. These results provided valuable insights into the phytochemical composition and therapeutic potential of\n                    <jats:italic>H. rosa-sinensis<\/jats:italic>\n                    . Additional investigations into the bioactive components of\n                    <jats:italic>H. rosa-sinensis<\/jats:italic>\n                    might result in the creation of new antibacterial and anticancer medicines for use in a range of medical applications.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/2722306","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T23:33:39Z","timestamp":1745883219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Phytochemical Analysis, Antibacterial, and Antitumor Potential of\n                    <i>Hibiscus rosa-sinensis<\/i>\n                    Linn"],"prefix":"10.1155","volume":"2025","author":[{"given":"Neelma","family":"Munir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheza Ayaz","family":"Khilji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajal","family":"Rasool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anam","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8954-6282","authenticated-orcid":false,"given":"Zahoor Ahmad","family":"Sajid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511753312.005"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.52711\/0975-4385.2023.00006"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11101331"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.26442\/20751753.2022.9.201944"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.36948\/ijfmr.2023.v05i01.1667"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.2174\/1871530323666230522113405"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.36948\/ijfmr.2022.v04i06.1242"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.36948\/ijfmr.2022.v04i05.908"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000444063"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.5772\/60050"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-90390-5.00010-4"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-19-0872-9_12"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi.22-00043"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.21608\/ajps.2020.118379"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.14302\/issn.2575-7881.jdrr-20-3267"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2023.2213341"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.46784\/eavm.v15i1.294"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.17265\/2328-2150\/2016.10.003"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1063\/5.0027486"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.58996\/hmj.v5i1.37"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.18203\/2320-6012.ijrms20170139"},{"key":"e_1_2_10_22_2","first-page":"865","article-title":"Clinical and Susceptibility Profile From Diabetic Foot Patients in Tertiary Care Hospital","volume":"2","author":"Kaur N.","year":"2014","journal-title":"Scholars Journal of Applied Medical Sciences"},{"key":"e_1_2_10_23_2","first-page":"65","article-title":"Bacterial Profile of Diabetic Foot Ulcer-Study From Western India","volume":"6","author":"Ibrahim A. J.","year":"2016","journal-title":"International Journal of Health Sciences & Research"},{"key":"e_1_2_10_24_2","first-page":"403","volume-title":"Methods in Molecular Biology","author":"Desbois A. P.","year":"2015"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir1061"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1537-2197.1977.tb15713.x"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.61.6.1031"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1078\/094471103321659834"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/np50024a005"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2018.05.031"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1590\/s1517-83822007000200034"},{"key":"e_1_2_10_33_2","first-page":"87","article-title":"Screening of Antitumor Activity of Datura metel L. Using Potato Disc Bioassay","volume":"2","author":"Islam M. S.","year":"2008","journal-title":"Plant Environment and Development"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(95)01305-9"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2017.604.132"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.31254\/jahm.2018.4308"},{"key":"e_1_2_10_37_2","first-page":"1210","article-title":"Preliminary Bioactive Compounds Screening and Antibacterial Activity of Methanolic Extract of Hibiscus rosa-sinensis Against Selected Skin Pathogens","volume":"5","author":"Hemarana K.","year":"2014","journal-title":"Research Journal of Pharmaceutical, Biological and Chemical Sciences"},{"key":"e_1_2_10_38_2","first-page":"15","article-title":"Study of Fungal and Bacterial Infections of the Diabetic Foot","volume":"45","author":"Chincholikar D. A.","year":"2002","journal-title":"Indian Journal of Pathology & Microbiology"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0953-6205(03)00107-9"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90043-5"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.37.060186.002453"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.58.030603.123630"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.2000.00808.x"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.1159\/000011830"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1549"},{"key":"e_1_2_10_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.182.14.3885-3895.2000"},{"key":"e_1_2_10_47_2","doi-asserted-by":"publisher","DOI":"10.1515\/jcim-2023-0290"},{"key":"e_1_2_10_48_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ecolsys.36.102003.152636"},{"key":"e_1_2_10_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00968a057"},{"key":"e_1_2_10_50_2","first-page":"1251","article-title":"Challenges and Opportunities in Drug Discovery From Plants","volume":"4","author":"Jachak S. M.","year":"2007","journal-title":"Current Science"},{"key":"e_1_2_10_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-58439-3"}],"container-title":["Scientifica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/2722306","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/sci5\/2722306","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/2722306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:08:01Z","timestamp":1772968081000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/sci5\/2722306"}},"subtitle":[],"editor":[{"given":"Mohamed","family":"Addi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/sci5\/2722306"],"URL":"https:\/\/doi.org\/10.1155\/sci5\/2722306","archive":["Portico"],"relation":{},"ISSN":["2090-908X","2090-908X"],"issn-type":[{"value":"2090-908X","type":"print"},{"value":"2090-908X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2722306"}}