{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T17:18:16Z","timestamp":1779297496581,"version":"3.51.4"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":58,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/10940-1"],"award-info":[{"award-number":["2017\/10940-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/13096-2"],"award-info":[{"award-number":["2021\/13096-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2018\/15083-2"],"award-info":[{"award-number":["2018\/15083-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["23430\/2021-1"],"award-info":[{"award-number":["23430\/2021-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["310049\/2022-9"],"award-info":[{"award-number":["310049\/2022-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/04279"],"award-info":[{"award-number":["UID\/04279"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["2018-06489"],"award-info":[{"award-number":["2018-06489"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Odontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin\u2013pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative dentinogenesis in vital pulp therapies, especially in immature permanent teeth. This study evaluated the effect of flavonoids on odontoblast secretory activity and the expression of dentinogenesis markers. The effect of flavonoids was evaluated on phenotypic mineralization markers (alkaline phosphatase (ALP) activity and mineralized nodule deposition) by colorimetric assays and on the expression of\n                    <jats:italic>Alpl<\/jats:italic>\n                    ,\n                    <jats:italic>Mmp2<\/jats:italic>\n                    ,\n                    <jats:italic>Mmp9<\/jats:italic>\n                    ,\n                    <jats:italic>Dmp1<\/jats:italic>\n                    , and\n                    <jats:italic>Dspp<\/jats:italic>\n                    genes in odontoblast\u2010like cells by quantitative polymerase chain reaction. Most of the flavonoids did not show toxicity between 100 and 25\u2009\u03bcM. In distinct concentrations, epigallocatechin gallate (EGCG), taxifolin, myricetin, quercetin, and kaempferol stimulated the activity of ALP and increased mineralized nodule deposition. However, the highest effect on those phenotypic markers was observed after EGCG and taxifolin treatments. Then, they were selected for evaluation of gene expression. mRNA levels of\n                    <jats:italic>Dmp1<\/jats:italic>\n                    and\n                    <jats:italic>Dspp<\/jats:italic>\n                    highly increased with taxifolin treatment, and\n                    <jats:italic>Alpl<\/jats:italic>\n                    expression was increased for both taxifolin and EGCG groups, without difference between them.\n                    <jats:italic>Mmp2<\/jats:italic>\n                    and\n                    <jats:italic>Mmp9<\/jats:italic>\n                    expression was not affected by these flavonoids. In conclusion, EGCG and taxifolin positively affect phenotypic mineralization markers; in particular, taxifolin highly stimulates early\u2010 and late\u2010stage dentinogenesis genes.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/3233536","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T23:44:26Z","timestamp":1741736666000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["EGCG and Taxifolin Modulate Secretory Activity and Expression of Dentinogenesis Markers in Odontoblast\u2010Like Cells"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2575-279X","authenticated-orcid":false,"given":"Cristiane","family":"Duque","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1104-9283","authenticated-orcid":false,"given":"Rafaela Laruzo","family":"Rabelo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5004-7489","authenticated-orcid":false,"given":"Gabriela Pacheco de Almeida","family":"Braga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5748-5040","authenticated-orcid":false,"given":"Igor Paulino","family":"Mendes Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eduarda","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0622-8236","authenticated-orcid":false,"given":"Daniela Alvim","family":"Chrisostomo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2846-2325","authenticated-orcid":false,"given":"Josimeri","family":"Hebling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7455-6867","authenticated-orcid":false,"given":"Carlos Alberto","family":"de Souza Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8969-9043","authenticated-orcid":false,"given":"Anuradha","family":"Prakki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3705-0971","authenticated-orcid":false,"given":"Lu\u00eds Octavio","family":"Regasini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,28]]},"reference":[{"key":"e_1_2_10_1_2","first-page":"170","article-title":"Guideline on Pulp Therapy for Primary and Young Permanent Teeth","volume":"30","author":"American Academy on Pediatric Dentistry Clinical Affairs Committee-Pulp Therapy Subcommittee","year":"2008","journal-title":"Pediatric Dentistry"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40368-015-0191-y"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(82)80092-7"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/cga.12169"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.12.012"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2009.09.006"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.5037\/jomr.2014.5403"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512467034"},{"key":"e_1_2_10_9_2","first-page":"17055","article-title":"Clinical Outcome of Direct Pulp Capping With MTA or Calcium Hydroxide: A Systematic Review and Meta-Analysis","volume":"8","author":"Zhu C.","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2016.41"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113086"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2013.03.001"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2013.09.009"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2019.108228"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-020-00427-2"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107bor-2021.vol35.0140"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2020.105034"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/09636897211034452"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomimetics8010075"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2022.105413"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.3109\/03008209809002442"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107BOR-2017.vol31.0104"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2018.08.297"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205880200"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12426"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26061580"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2006.06.005"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.06.002"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmbbm.2021.104392"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/J.BCP.2003.11.009"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2020.112466"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/162750"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2018.01.025"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2021.105268"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004770-200204000-00013"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.3290\/j.jad.a42548"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-021-03213-5"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20236042"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/jfb14030139"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/ma12071184"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.135.3.8070395"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00082.2005"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01476.x"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-020-00313-1"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/jfb13040305"},{"key":"e_1_2_10_46_2","doi-asserted-by":"publisher","DOI":"10.1590\/1678-7757-2022-0282"},{"key":"e_1_2_10_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2024.105424"},{"key":"e_1_2_10_48_2","unstructured":"DuqueC. Microbiological and Anti-Inflammatory Evaluation and Capacity of Mineralization Induction of Flavonoids as Agents for Application in Dentistry 2021 Universidade Estadual Paulista (UNESP) https:\/\/repositorio.unesp.br\/entities\/publication\/e6e77197-abc8-41d5-bb0f-d1a126668bf5\/full Thesis Microbiology."},{"key":"e_1_2_10_49_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241814056"}],"container-title":["Scientifica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/3233536","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/sci5\/3233536","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/3233536","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:10:24Z","timestamp":1772968224000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/sci5\/3233536"}},"subtitle":[],"editor":[{"given":"Sukhamoy","family":"Gorai","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/sci5\/3233536"],"URL":"https:\/\/doi.org\/10.1155\/sci5\/3233536","archive":["Portico"],"relation":{},"ISSN":["2090-908X","2090-908X"],"issn-type":[{"value":"2090-908X","type":"print"},{"value":"2090-908X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-07-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3233536"}}