{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:54:49Z","timestamp":1787162089289,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":181,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"\n The aim of this research is to investigate the antimicrobial characteristics of bacterial pigments. Pigments are vibrant metabolites generated by bacteria, offering defence against radiation, sunlight, stress and competing microorganisms. Bacterial pigments have various applications in cosmetics, pharmaceuticals and food sectors due to their antimicrobial, anticancer and antioxidant properties. In this cross\u2010sectional in vitro study, we used 30 isolates each of\n Enterococcus faecalis<\/jats:italic>\n ,\n Klebsiella<\/jats:italic>\n spp. and\n Candida<\/jats:italic>\n spp. isolated from human urine, blood and pus samples to study the antimicrobial effect of pigments produced by\n Serratia marcescens<\/jats:italic>\n (prodigiosin),\n Chromobacterium violaceum<\/jats:italic>\n (violacein) and\n Pseudomonas aeruginosa<\/jats:italic>\n (pyocyanin). At the suitable pH and temperature, pigment\u2010producing bacteria were mass\u2010cultured in nutrient broth. Pigments were extracted from\n S. marcescens<\/jats:italic>\n and\n C. violaceum<\/jats:italic>\n cultures using acetone and methanol. Pyocyanin from\n P. aeruginosa<\/jats:italic>\n was extracted by using hydrochloric acid and chloroform. Extracted pigments were dried and characterised by mass spectrometry. The antimicrobial activity of the crude pigments was determined by the agar dilution method using Muller Hinton agar (MHA) plates for bacterial isolates, and Sabouraud\u2019s Dextrose agar (SDA) for\n Candida<\/jats:italic>\n spp.\n E. faecalis<\/jats:italic>\n ATCC 29212,\n K. pneumonia<\/jats:italic>\n ATCC 700603 and\n C. albicans<\/jats:italic>\n ATCC 14053 were used as standards. The minimum concentration of the pigment that supressed the growth of microorganism on the MHA\/SDA agar plate was considered as minimum inhibitory concentration (MIC). Data were analysed using the Chi\u2010square statistical method.\n E. faecalis<\/jats:italic>\n , which were resistant to teicoplanin, vancomycin, norfloxacin and penicillin, were found susceptible to pyocyanin (\n p<\/jats:italic>\n < 0.05). Multidrug\u2010resistant\n Klebsiella<\/jats:italic>\n spp. were susceptible to pyocyanin, violacein and prodigiosin (\n p<\/jats:italic>\n < 0.05).\n Candida<\/jats:italic>\n spp. resistant to caspofungin, micafungin, showed susceptibility to prodigiosin, and those isolates resistant to amphotericin B showed susceptibility to pyocyanin (\n p<\/jats:italic>\n < 0.05). Among all clinical isolates,\n Klebsiella<\/jats:italic>\n spp. from urine exhibited the highest resistance to the pigments studied. Among the pigments studied, violacein and prodigiosin exhibited superior antimicrobial and antifungal properties. Therefore, violacein and prodigiosin could serve as an alternative antimicrobial substance for addressing multidrug\u2010resistant pathogenic microorganisms.\n <\/jats:p>","DOI":"10.1155\/sci5\/3951925","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T07:35:41Z","timestamp":1751355341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antimicrobial Effect of Common Bacterial Pigments on Clinically Significant Microorganisms"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2434-1300","authenticated-orcid":false,"given":"Afroz","family":"Salman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5370-0663","authenticated-orcid":false,"given":"Biranthabail","family":"Dhanashree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1252-507X","authenticated-orcid":false,"given":"Himani","family":"Kotian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-024-06834-4"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-018-1227-x"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11030614"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2024.106907"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2013.06.006"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9040739"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fsufs.2020.00100"},{"key":"e_1_2_12_8_2","first-page":"211","article-title":"Antimicrobial Evaluation of Bioactive Pigment from Salinococcus Sp Isolated from Nellore Sea Coast","volume":"13","author":"Srilekha V.","year":"2017","journal-title":"International Journal of Biotechnology & Biochemistry"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9010011"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008150"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.202200286"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2016.1195380"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162412"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0253445"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.4014\/mbl.1901.01006"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.22207\/jpam.16.2.28"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.7324\/JAPS.2012.2823"},{"key":"e_1_2_12_18_2","first-page":"1","article-title":"Extraction, Purification and Characterization of Pyocyanin by Pseudomonas Aeuroginosa and Evaluation for its Antimicrobial Activity","volume":"6","author":"Devnath P.","year":"2017","journal-title":"International Research Journal of Biological Sciences"},{"key":"e_1_2_12_19_2","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Anaerobically","author":"Clsi","year":"2023"},{"key":"e_1_2_12_20_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing. 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