{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:08:06Z","timestamp":1789099686803,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":327,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004944","name":"Naresuan University","doi-asserted-by":"publisher","award":["R2567C034"],"award-info":[{"award-number":["R2567C034"]}],"id":[{"id":"10.13039\/501100004944","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004944","name":"Naresuan University","doi-asserted-by":"publisher","award":["R2566C053"],"award-info":[{"award-number":["R2566C053"]}],"id":[{"id":"10.13039\/501100004944","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004944","name":"Naresuan University","doi-asserted-by":"publisher","award":["R2569C007"],"award-info":[{"award-number":["R2569C007"]}],"id":[{"id":"10.13039\/501100004944","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Ko-klan<\/jats:italic>\n                    is a traditional Thai herbal medicine that is officially recognized for treating muscle pain; however, its mechanism of action has not been characterized. This study investigated the antioxidant and anti\u2010inflammatory properties of three Ko\u2010klan remedy formulations and identified potential bioactive markers using molecular docking. Ko\u2010klan formulations were extracted using decoction, maceration, ultrasound\u2010assisted extraction (UAE), and microwave\u2010assisted extraction (MAE), with ethanol as the solvent. Phytochemical analyses were performed to determine the total phenolic content (TPC) and total flavonoid content (TFC), with antioxidant activity evaluated using the DPPH, ABTS, and FRAP assays. The anti\u2010inflammatory effects were assessed by gene expression analysis (TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , iNOS, COX\u20102, and IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ) and inhibition of nitric oxide production, and lipoxygenase (LOX) activity. LC\u2010ESI\u2010QTOF\u2010MS\/MS identified 175 compounds, with molecular docking performed against 5\u2010LOX. Formulation\u20103 exhibited notable bioactivity, with a TPC of up to 1.08\u2009g GAE\/g and a TFC of 7.30\u2009mg QE\/g, and IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 51.8\u2009\u03bcg\/mL for DPPH and 92.3\u2009\u03bcg\/mL for ABTS assays, 7 to 10 fold than the Trolox standard. Anti\u2010inflammatory activity showed comparable inhibition of nitric oxide production to the L\u2010NAME standard and effective LOX inhibition at 62.7\u2009\u03bcg\/mL. The MAE\u2010based Ko\u2010klan remedy extract significantly suppressed LPS\u2010induced inflammatory gene expression, comparable to dexamethasone. Molecular docking showed that caffeoyl quinic acid and brazilin were potent 5\u2010LOX inhibitors with binding energies of \u221210.14 and \u221210.24\u2009kcal\/mol, respectively. Results demonstrate that Ko\u2010klan remedies, particularly formulation\u20103, possessed significant antioxidant and anti\u2010inflammatory activities, with phytochemical richness, effective suppression of inflammatory mediators, and potential bioactive markers such as caffeoyl quinic acid and brazilin, thereby supporting their traditional use and providing a scientific basis for further therapeutic development.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/4361994","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T05:13:03Z","timestamp":1764047583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Potential of Ko\u2010Klan Traditional Thai Remedy for Evaluation of Antioxidant and RT\u2010PCR Anti\u2010Inflammatory Activities"],"prefix":"10.1155","volume":"2025","author":[{"given":"Sutthichat","family":"Kerdphon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pariya","family":"Atawong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukanya","family":"Reanpang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phanupong","family":"Changtor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nopawit","family":"Khamto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gorawit","family":"Yusakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitra","family":"Nuengchamnong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kittisak","family":"Buddhachat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9751-2422","authenticated-orcid":false,"given":"Jira","family":"Jongcharoenkamol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"e_1_2_11_1_2","volume-title":"Guideline for the Use of the National List of Essential Medicines: Herbal Medicines B.E. 2564","author":"Thai Food and Drug Administration","year":"2023"},{"key":"e_1_2_11_2_2","first-page":"24","article-title":"In Vitro Antioxidant Potential of Mallotus repandus (Willd.) 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