{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T22:39:14Z","timestamp":1785278354052,"version":"3.55.0"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":239,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    As potent therapeutic agents, the pharmacological potentials of natural substances have been the subject of recent research. Around the world, numerous tribes and ethnic communities have long used\n                    <jats:italic>Annona reticulata<\/jats:italic>\n                    Linn. (Family:\n                    <jats:italic>Annonaceae<\/jats:italic>\n                    ) to treat variety of illnesses. Diverse therapeutic effects, including anthelmintic, antipyretic, antihyperglycemic, antiulcer, and antinociceptive properties are demonstrated by the plant. The current study assessed the plant\u2019s antioxidant, cytotoxic, and anticancer activities along with identifying probable responsible compounds for these effects via GC\u2010MS analysis. The antioxidant potential was assessed using six assays. In DPPH and nitric oxide radical scavenging tests, good antioxidant property was demonstrated by test fraction with observed IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 83.72 and 107.92\u2009\u03bcg\/mL. The extract\u2019s reducing potential was found to enhance with enhancing concentration. The 139.5\u2009\u00b1\u20097.21\u2009mg ascorbic acid equivalent\/g extract was obtained as the total antioxidant capacity value. The calculated total phenolic and flavonoid contents values for the extract were 69.73\u2009\u00b1\u20090.26\u2009mg gallic acid equivalent\/g extract and 93.62\u2009\u00b1\u20090.15\u2009mg quercetin equivalent\/g extract. The extract showed promising cytotoxic property in the brine shrimp lethality bioassay. In this study, the observed LC\n                    <jats:sub>50<\/jats:sub>\n                    value for the extract was 114.12\u2009\u03bcg\/mL; while the value for vincristine sulfate was 1.63\u2009\u03bcg\/mL. The extract\u2019s in vivo anticancer activity against EAC cell line was also remarkable. The 400\u2009mg\/kg of body weight dose of methanolic leaf extract showed tumor weight and EAC cell number values of 3.20\u2009\u00b1\u20090.20\u2009g and 8.40\u2009\u00b1\u20090.51\u2009cells\/mL; both of these values were lower than the values obtained from the standard drug 5\u2010fluorouracil.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/5166065","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T06:20:22Z","timestamp":1756448422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of Antioxidant, Cytotoxic, and Anticancer Activities of Methanol Extract of\n                    <i>Annona reticulata<\/i>\n                    Linn. Leaves"],"prefix":"10.1155","volume":"2025","author":[{"given":"Tasnia Binte Bari","family":"Kabbo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahim Shahrier","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Sohel","family":"Rana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8779-9550","authenticated-orcid":false,"given":"Pritesh Ranjan","family":"Dash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf9029892"},{"key":"e_1_2_10_2_2","first-page":"570","article-title":"GC-MS Analysis of Phytocomponents in the Methanolic Extract of Justicia wynaadensis (Nees) T. anders","volume":"3","author":"Ponnamma S. U.","year":"2012","journal-title":"International Journal of Pharma and Bio Sciences"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0024-3205(03)00259-5"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27154989"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.4274\/tjps.80299"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(91)90110-y"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(93)90567-y"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-8741(99)00161-0"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00716"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.5586\/asbp.2012.010"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.5530\/ijper.51.2.39"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/pr11082248"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.22271\/flora.2016.v4.i6c.03"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf0100907"},{"key":"e_1_2_10_15_2","first-page":"83","article-title":"In Vitro Nitric Oxide Scavenging Activity of Methanol Extracts of Three Bangladeshi Medicinal Plants","volume":"1","author":"Parul R.","year":"2013","journal-title":"The Pharma Innovation"},{"key":"e_1_2_10_16_2","first-page":"99","article-title":"Determination of Total Phenolic Content and Total Antioxidant Activity in Locally Consumed Food Stuffs in Moodbidri, Karnataka, India","volume":"6","author":"Kamath S. D.","year":"2015","journal-title":"Advances in Applied Science Research"},{"key":"e_1_2_10_17_2","first-page":"260","article-title":"Determination of Total Phenolic Content, Total Flavonoid Content and Total Antioxidant Capacity in Some Endemic Sideritisl.(Lamiaceae) Species Grown in Turkey","volume":"11","author":"Sevindik E.","year":"2021","journal-title":"European Journal of Biological Research"},{"key":"e_1_2_10_18_2","first-page":"53","article-title":"Determination of Total Phenolic Content, Total Flavonoid Content and Antioxidant Activity of Various Organic Crude Extracts of Licuala spinosa Leaves from Sabah, Malaysia","volume":"11","author":"Khan M. S.","year":"2018","journal-title":"ASM Science Journal"},{"key":"e_1_2_10_19_2","article-title":"Assessment of Antioxidant Activity of Different Extracts of Annona Reticulate (L.)","volume":"9","author":"Pathak K.","year":"2018","journal-title":"International Journal of Pharmaceutical Sciences and Research"},{"key":"e_1_2_10_20_2","first-page":"1698","article-title":"Spectroscopic Determination of Total Phenol and Flavonoid Contents of Ipomoea carnea","volume":"2","author":"Khatiwora E.","year":"2010","journal-title":"International Journal of ChemTech Research"},{"key":"e_1_2_10_21_2","article-title":"Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants","volume":"41","author":"Waghulde S.","year":"2019","journal-title":"Proceedings"},{"key":"e_1_2_10_22_2","article-title":"Cytotoxicity Evaluation of Jordanian Wild Plants Using Brine Shrimp Lethality Test","volume":"8","author":"Tawaha K. A.","year":"2006","journal-title":"Jordan Journal of Applied Science Natural Sciences"},{"key":"e_1_2_10_23_2","article-title":"Brine Shrimp Lethality Assay of the Ethanolic Extracts of Three Selected Species of Medicinal Plants From Iligan City, Philippines","volume":"1","author":"Olowa L. F.","year":"2013","journal-title":"Mortality"},{"key":"e_1_2_10_24_2","first-page":"165","article-title":"Evaluation of Anticancer Activity of Methanol Extract of Monstera deliciosa in EAC Induced Swiss Albino Mice","volume":"7","author":"Prosanta P.","year":"2015","journal-title":"International Journal of Toxicological and Pharmacological Research"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.5455\/spatula.20120501080940"},{"key":"e_1_2_10_26_2","first-page":"1503","article-title":"Anticancer and Antioxidant Effect of Acanthophoraspicifera Against EAC Induced Carcinoma in Mice","volume":"5","author":"Lavakumar V.","year":"2012","journal-title":"Journal of Pharmacy Research"},{"key":"e_1_2_10_27_2","first-page":"340","article-title":"Evaluation of Anti-Cancer Activity of Ruellia tuberosa on EAC Induced Solid Tumor","volume":"4","author":"Reddy V. N.","year":"2013","journal-title":"International Journal of Phytopharmacology"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.3109\/13880201003792592"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-015-1380-2"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-020-03029-9"},{"key":"e_1_2_10_31_2","first-page":"2178","article-title":"Determination of Phytoconstituents in Annona reticulata Linn. Methanolic Leaf Extract Using GCMS","volume":"11","author":"Sankpal M. M.","year":"2022","journal-title":"World Journal of Pharmaceutical Research"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.117818"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.13057\/biodiv\/d241033"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.2174\/1871520618666180821113830"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-824412-8.00016-3"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.5530\/pj.2021.13.49"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2018.08.032"},{"key":"e_1_2_10_38_2","first-page":"5755","article-title":"Thymol Derivatives with Antibacterial and Cytotoxic Activity From the Aerial Parts of Ageratinaadenophora","volume":"11","author":"Zhang M.","year":"2021","journal-title":"Royal Society of Chemistry Advances"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.13005\/bpj\/2601"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389557521666210105123320"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2017.607.007"},{"key":"e_1_2_10_42_2","first-page":"1","article-title":"Cyclopropane Derivatives and Their Diverse Biological Activities","author":"Sala\u00fcn J.","year":"2000","journal-title":"Small Ring Compounds in Organic Synthesis"},{"key":"e_1_2_10_43_2","first-page":"212","article-title":"Pharmacological Application of Thiophene Derivatives","volume":"8","author":"Bhagwat J. K.","year":"2023","journal-title":"International Journal of Multidisciplinary Research"},{"key":"e_1_2_10_44_2","article-title":"Biological Oxidations and Antioxidant Activity of Natural Products","author":"Nunes P. X.","year":"2012","journal-title":"Phytochemicals as nutraceuticals-Global Approaches to Their Role in Nutrition and Health"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.4314\/ajtcam.v5i1.31258"},{"key":"e_1_2_10_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbp.2010.04.008"},{"key":"e_1_2_10_47_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-40422010001000033"},{"key":"e_1_2_10_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-12-221"},{"key":"e_1_2_10_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2007.09.007"},{"key":"e_1_2_10_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(99)00120-4"},{"key":"e_1_2_10_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2004.07.028"},{"key":"e_1_2_10_52_2","first-page":"86","article-title":"Evaluation of Antioxidant Activity of Two Indian Medicinal Plants","volume":"3","author":"Padmaja M.","year":"2011","journal-title":"Journal of Phytology"},{"key":"e_1_2_10_53_2","first-page":"90","article-title":"Phytochemicals, Antimicrobial and Antioxidant Properties of Annona reticulata Linn","volume":"6","author":"Jayaprakash A.","year":"2017","journal-title":"Journal of Academia and Industrial Research"},{"key":"e_1_2_10_54_2","doi-asserted-by":"publisher","DOI":"10.3742\/OPEM.2008.8.3.260"},{"key":"e_1_2_10_55_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-971236"},{"key":"e_1_2_10_56_2","doi-asserted-by":"publisher","DOI":"10.3329\/bjm.v29i2.28439"},{"key":"e_1_2_10_57_2","doi-asserted-by":"publisher","DOI":"10.2174\/1570178616666190724123004"},{"key":"e_1_2_10_58_2","first-page":"96","article-title":"Practical Murine Hematopathology: A Comparative Review and Implications for Research","volume":"65","author":"O\u2019Connell K. E.","year":"2015","journal-title":"Comparative Medicine"},{"key":"e_1_2_10_59_2","doi-asserted-by":"publisher","DOI":"10.1080\/15287398609530859"},{"key":"e_1_2_10_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.04.046"},{"key":"e_1_2_10_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaim.2021.05.010"},{"key":"e_1_2_10_62_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.141542.2"},{"key":"e_1_2_10_63_2","doi-asserted-by":"publisher","DOI":"10.5923\/j.als.20120202.01"}],"container-title":["Scientifica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/5166065","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/sci5\/5166065","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/sci5\/5166065","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:03:54Z","timestamp":1772967834000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/sci5\/5166065"}},"subtitle":[],"editor":[{"given":"Hicham","family":"Harhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/sci5\/5166065"],"URL":"https:\/\/doi.org\/10.1155\/sci5\/5166065","archive":["Portico"],"relation":{},"ISSN":["2090-908X","2090-908X"],"issn-type":[{"value":"2090-908X","type":"print"},{"value":"2090-908X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2025-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5166065"}}