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Currently, endophytic fungi serve as alternate sources for the production of new bioactive chemicals with great efficacy. This study aimed to examine the antimigration and anti\u2010invasion capabilities of the endophytic fungus\n                    <jats:italic>Aspergillus aculeatus<\/jats:italic>\n                    extract, isolated from\n                    <jats:italic>Capsicum annuum<\/jats:italic>\n                    L., utilizing\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in silico<\/jats:italic>\n                    methods. This study isolated the endophytic fungus\n                    <jats:italic>A. aculeatus<\/jats:italic>\n                    from the leaves of\n                    <jats:italic>C. annuum<\/jats:italic>\n                    L. LC\u2013MS analysis revealed fifty\u2010five active components within the extract. Ten compounds exhibited favorable results in the\n                    <jats:italic>in silico<\/jats:italic>\n                    assessment. Computational predictions indicate that tajixanthone methanoate (\u22128.80\u2009kcal\/mol) and aspernigerin (\u221212.95\u2009kcal\/mol) exhibited high binding affinity against MMP\u20102. The\n                    <jats:italic>A. aculeatus<\/jats:italic>\n                    extract demonstrated antiproliferative activity with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 286.36\u2009\u00b1\u2009122.57\u2009\u03bcg\/mL. The extract, at noncytotoxic concentrations, reduced the migration and invasion of A549 cells in a dose\u2010dependent manner. Furthermore,\n                    <jats:italic>A. aculeatus<\/jats:italic>\n                    extract demonstrated a marked reduction in MMP\u20102 activity. According to these results, the compounds may serve as antimigration and anti\u2010invasion agents by inhibiting the MMP\u20102 protein. The results demonstrated that\n                    <jats:italic>A. aculeatus<\/jats:italic>\n                    extract derived from\n                    <jats:italic>C. annuum<\/jats:italic>\n                    L. inhibited A549 cell migration and invasion via reducing MMP\u20102 activity. The findings indicated that\n                    <jats:italic>A. aculeatus<\/jats:italic>\n                    extract derived from\n                    <jats:italic>C. annuum<\/jats:italic>\n                    L. may be utilized for the treatment of lung cancer.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/5676577","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T10:03:13Z","timestamp":1755770593000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antimigration and Anti\u2010Invasion Properties of\n                    <i>Aspergillus aculeatus<\/i>\n                    Extract, an Endophyte Isolated From\n                    <i>Capsicum annuum<\/i>\n                    L. on Non\u2013Small\u2010Cell Lung Cancer Cells:\n                    <i>In Vitro<\/i>\n                    Experiments and\n                    <i>In Silico<\/i>\n                    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