{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:36:51Z","timestamp":1772987811547,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":317,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>Marek\u2019s disease caused by Marek\u2019s disease virus (MDV) serotype 1 is an economically important neoplastic disease of poultry. Diagnosis of this disease is usually based on clinical signs, postmortem lesions, and diagnostic tests like cytology, histopathology, and molecular\u2010based methods. However, there might be the problem of inaccurate diagnosis in Nigeria. Employment of gross pathology, histopathology, immunohistochemistry, and polymerase chain reaction (PCR) coupled with sequence analysis provides a reliable approach to arrive at precise confirmatory diagnosis of the disease. Therefore, visceral organs including liver, proventriculus intestine, spleen, and heart samples were collected at postmortem examinations from two pullets suspected of having Marek\u2019s disease. Histopathology and immunohistochemistry, PCR amplification of a 576\u2009bp fragment of the MDV glycoprotein L (gL) gene, and sequence analysis were employed in this diagnostic approach. Histopathological examinations of the liver, heart and proventriculus showed neoplastic pleomorphic cellular infiltration comprising lymphoblasts, lymphocytes, macrophages, and heterophils which correspond with the pathology of Marek\u2019s disease. Liver samples were positive for the virus using PCR. Sequence analysis based on phylogenetic tree reconstruction revealed the positive MDV. MDV sequences from this study clustered with MDV serotype 1 sequences retrieved from the GenBank. This approach provides a reliable and precise diagnosis of Marek\u2019s disease in chickens which is applicable to other avian diseases.<\/jats:p>","DOI":"10.1155\/sci5\/5848535","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T10:37:12Z","timestamp":1763116632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathology and Molecular Diagnosis of Marek\u2019s Disease Virus in Chickens in Nigeria"],"prefix":"10.1155","volume":"2025","author":[{"given":"Olusegun Adesina","family":"Fagbohun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiki-Raji Comfort","family":"Oluladun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theophilus Aghogho","family":"Jarikre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olugbenga Olayinka","family":"Alaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8996-8610","authenticated-orcid":false,"given":"Ademola Adetokunbo","family":"Oyagbemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rofiat Damilola","family":"Adesina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oluwaseun Olanrewaju","family":"Esan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9960-3615","authenticated-orcid":false,"given":"Olumide Odunayo","family":"Akinniyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moses Olusola","family":"Adetona","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9486-2861","authenticated-orcid":false,"given":"Temidayo Olutayo","family":"Omobowale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ufuoma Joan","family":"Mamoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9310-6511","authenticated-orcid":false,"given":"Ishmael Festus","family":"Jaja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"key":"e_1_2_9_1_2","article-title":"The Structure and Importance of the Commercial and Village-based Poultry Industry in Nigeria","author":"Adene D. 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