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These diseases have limited treatment options; therefore, drug discovery remains a critical priority. This study evaluated the leaf extract of\n                    <jats:italic>Annona muricata<\/jats:italic>\n                    L., which contains quercetin, formulated in silica nanoparticles against\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    species. The anti\u2010\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    effect of the nanoformulation was determined by its MIC and percentage viability. Scanning electron microscopy confirmed the nanocompound\u2010directed effect on trophozoites and cysts. The nanoquercetin exhibited anti\u2010\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    activity, showing greater effectiveness against the trophozoites of\n                    <jats:italic>Acanthamoeba polyphaga<\/jats:italic>\n                    ATCC 30461 (AP) (MIC &gt; 2\u2009mg\/mL) compared to cysts (MIC &gt;\u20098\u2009mg\/mL) and against\n                    <jats:italic>A. triangularis<\/jats:italic>\n                    WU19001 (AT) trophozoites (MIC &gt;\u20094\u2009mg\/mL) compared to cysts (MIC &gt;\u20098\u2009mg\/mL). It also showed reduced activity against\n                    <jats:italic>Acanthamoeba castellanii<\/jats:italic>\n                    ATCC 30010 (AC3) trophozoites (MIC &gt;\u20094\u2009mg\/mL) compared to cysts (MIC &gt;\u20098\u2009mg\/mL) and\n                    <jats:italic>A. castellanii<\/jats:italic>\n                    ATCC50739 (AC5) trophozoites (MIC &gt;\u20094\u2009mg\/mL) compared to cysts (MIC &gt;\u20098\u2009mg\/mL). The anti\u2010\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    activity of\n                    <jats:italic>A. muricata<\/jats:italic>\n                    on all four species was compared to chlorhexidine, which had MIC values of 0.008\u2009mg\/mL for AC3 and AT trophozoites and 0.016\u2009mg\/mL for AC5 and AP trophozoites. For cysts, chlorhexidine exhibited MIC values of &gt;\u20090.0128\u2009mg\/mL for AC3 and AP, 0.032\u2009mg\/mL for AC5, and 0.064\u2009mg\/mL for AT. All MIC values demonstrated inhibition of\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    species with reduced viability exceeding 90%. SEM findings revealed morphological changes in\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    , indicating parasitic inhibition. Quercetin\u2010loaded silica nanoparticles demonstrated anti\u2010\n                    <jats:italic>Acanthamoeba<\/jats:italic>\n                    activity with low cytotoxicity. This indicates the potential of quercetin as a nano\u2010enhanced natural therapeutic or contact lens\u2010care agent. This study is limited by its in vitro design, small parasite strain panel, and potential nanoparticle variability. This highlights the need for in vivo validation, ocular\/contact lens model testing, and formulation optimization for improved cysticidal efficacy.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/5902580","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:49:40Z","timestamp":1788270580000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti\u2010\n                    <i>Acanthamoeba<\/i>\n                    Activities of Silica Nanoformulated Quercetin Compound Derived From\n                    <i>Annona muricata<\/i>\n                    L. 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