{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:36:06Z","timestamp":1772994966197,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":60,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The discovery of alternative therapies for illnesses due to multidrug\u2010resistant (MDR) bacteria is emerging as a global health crisis. This study aimed to assess the antibacterial efficacy of\n                      <jats:italic>Allanblackia gabonensis<\/jats:italic>\n                      extracts, both alone and in conjunction with antibiotics, against MDR\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The extracts were subjected to phytochemical analysis using both qualitative and quantitative standard tests. The microdilution assay was used to evaluate the antibacterial properties and antibiotic resistance\u2010modifying potential of the extracts. The interaction effect between the antibiotics and extracts was determined by checkerboard assay. Catalase activity and lipid peroxidation were assessed by measuring the foam height and malondialdehyde concentration, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The extracts exhibited minimum inhibitory concentrations (MICs) ranging from 16 to 2048 \u03bcg\/mL. The hexane extracts of the leaves (HLE) and bark (HBE) displayed the highest antibacterial activity, with MIC \u2264\u200932\u2009 \u03bcg\/mL against at least two investigated isolates. Furthermore, HLE decreased catalase activity and increased lipid peroxidation in\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      D130. The extracts at their sub\u2010inhibitory concentration (MIC\/8) enhanced the activity of antibiotics, mainly aminoglycosides (amikacin, streptomycin, and gentamicin), by 2\u2010 to 256\u2010fold against selected MDR\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      . Among these extracts, the hexane and methanol bark extracts exhibited synergy (\u2211FIC \u2264\u20090.5)) in combination with these antibiotics. All examined extracts contained alkaloids, phenols, and triterpenes. Moreover, dichloromethane\/methanol and methanol leaf extracts presented the highest phenolic content.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Overall, the leaf and bark hexane extracts of\n                      <jats:italic>A. gabonensis<\/jats:italic>\n                      could serve as candidates for the discovery of new antibiotics, while its bark extracts might be used in conjunction with antibiotics to manage infections involving multidrug\u2010resistant\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/sci5\/6070077","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:26:34Z","timestamp":1772983594000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Composition and Antipseudomonal Activity of\n                    <i>Allanblackia gabonensis<\/i>\n                    (Clusiaceae) Extracts Alone and With Antibiotics Against Drug\u2010Resistant Clinical Isolates"],"prefix":"10.1155","volume":"2026","author":[{"given":"C\u00e9line Brinda","family":"Sonfack","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7008-7453","authenticated-orcid":false,"given":"Aim\u00e9 Gabriel","family":"Fankam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda Ngueffo","family":"Tiwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0304-8264","authenticated-orcid":false,"given":"Michael Francis","family":"Kengne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4178-4967","authenticated-orcid":false,"given":"Armelle Tsafack","family":"Mbaveng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1070-1236","authenticated-orcid":false,"given":"Victor","family":"Kuete","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/3663315"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2018.11.013"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00477-5"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30753-3"},{"key":"e_1_2_13_5_2","unstructured":"CDC Antibiotic Resistance Threats in the United States 2019 https:\/\/www.cdc.gov\/antimicrobial-resistance\/media\/pdfs\/pseudomonas-aeruginosa-508.pdf."},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux020"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-08491-1_5"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/np200906s"},{"key":"e_1_2_13_9_2","volume-title":"African Flora to Fight Bacterial Resistance, Part II: The Best Source of Herbal Drugs and Pharmaceuticals","author":"Kuete V.","year":"2023"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.05.050"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021515"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000151267"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.6.556"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-015-0944-5"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2021.09.028"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1155\/sci5\/3403280"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0329771"},{"key":"e_1_2_13_18_2","article-title":"Flore du Congo Belge et du Ruanda Burundi","author":"Guttiferae (Clusiaceae) B. 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