{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:36:57Z","timestamp":1772987817230,"version":"3.50.1"},"reference-count":80,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":286,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Ruta montana<\/jats:italic>\n                    L. is a perennial medicinal herb traditionally used in Moroccan healing practices for infectious diseases, hyperglycemia, spasms, and fever. This work intends to evaluate the bioactive constituents of\n                    <jats:italic>Ruta montana<\/jats:italic>\n                    essential oil (RMEO) using the GC\u2013MS technique and demonstrate its antimicrobial, antioxidant, and antidiabetic properties via\n                    <jats:italic>in vitro<\/jats:italic>\n                    and molecular docking studies. The primary constituents of RMEO are 2\u2010undecanone (56.41%), limonene (10.28%), and 2\u2010nonanone (8.92%). The \u03b1\u2010amylase (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u2009455\u2009\u00b1\u20090.11\u2009\u03bcg\/mL) and \u03b1\u2010glucosidase (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u2009401\u2009\u00b1\u20090.04\u2009\u03bcg\/mL) enzymes were significantly inhibited by RMEO. Additionally, ferric reducing power (EC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20091188.61\u2009\u00b1\u20091.12\u2009\u03bcg\/mL) and the \u03b2\u2010carotene bleaching assay (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u2009206.21\u2009\u00b1\u20091.12\u2009\u03bcg\/mL) confirmed its strong antioxidant activity. The antimicrobial evaluation showed remarkable activity against\n                    <jats:italic>Micrococcus luteus<\/jats:italic>\n                    (IZ\u2009=\u200920.05\u2009\u00b1\u20090.98\u2009mm), followed by\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (IZ\u2009=\u200918.11\u2009\u00b1\u20091.11\u2009mm) and moderate anticandidal activity against\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    (IZ\u2009=\u200913.05\u2009\u00b1\u20090.21\u2009mm). Minimal inhibitory and bactericidal concentrations (MIC and MBC) ranged from 2.25 to 72\u2009mg\/mL, confirming both bactericidal and fungicidal effects. Besides,\n                    <jats:italic>in vitro<\/jats:italic>\n                    findings were supported by molecular docking and ADME analysis. The present investigation highlights the potent antibacterial, anticandidal, antioxidant, and antidiabetic abilities of RMEO, supporting its potential as a natural and safe therapeutic agent.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/6077249","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T14:07:33Z","timestamp":1760450853000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Profiling and Biological Activities of\n                    <i>Ruta montana<\/i>\n                    Volatile Oil:\n                    <i>In Vitro<\/i>\n                    and\n                    <i>In Silico<\/i>\n                    ADME\/Tox Assessment"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4507-6661","authenticated-orcid":false,"given":"Nesrine","family":"Benkhaira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0768-5914","authenticated-orcid":false,"given":"Naoufal El","family":"Hachlafi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0314-8484","authenticated-orcid":false,"given":"Amine","family":"Elbouzidi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3973-6694","authenticated-orcid":false,"given":"Mohamed","family":"Addi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6320-7381","authenticated-orcid":false,"given":"Saad Ibnsouda","family":"Koraichi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2923-9299","authenticated-orcid":false,"given":"Kawtar","family":"Fikri-Benbrahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnlssr.2024.02.002"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01398-5"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1210\/endrev\/bnad032"},{"key":"e_1_2_11_4_2","volume-title":"Flavonoids: Potential Therapeutic Agents by Their Antioxidant Capacity, Bioactive Compounds: Health Benefits and Potential Applications","author":"Hern\u00e1ndez-Rodr\u00edguez P.","year":"2018"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6002949"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22010070"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.02161"},{"key":"e_1_2_11_8_2","first-page":"65","article-title":"Essential Oils and Antimicrobial Effects","volume":"10","author":"Sezen S.","year":"2019","journal-title":"International Journal of Scientific Engineering and Research"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.34172\/jhp.2022.36"},{"key":"e_1_2_11_10_2","unstructured":"NajemM. BelaidiR. BouiamrineH. andIbijbijenJ. Usages en Pharmacop\u00e9e Traditionnelle au Moyen Atlas Central et Risques de la Rue de Montagne \u201cRuta montana L.\u201d Proceedings BIOSUNE\u20191 \u2013 2018 2019."},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/0972060x.2020.1829995"},{"key":"e_1_2_11_12_2","article-title":"Evaluation of the Antioxidant, Antifungal and Anticholinesterase Activity of the Extracts of Ruta montana L., Harvested From Souk-Ahras (North-East of Algeria) and Composition of Its Extracts by GC-MS","volume":"7","author":"Slougui N.","year":"2023","journal-title":"Tropical Journal of Natural Product Research"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1515\/jbcpp-2016-0030"},{"key":"e_1_2_11_14_2","first-page":"215","article-title":"Antioxidant and MDR Reversal Activity in Resistant Human Ovarian Cancer Cells of Methanolic Extract From Ruta montana Located in the North of Algeria","volume":"8","author":"Ali W. 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