{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:19:22Z","timestamp":1792293562858,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T00:00:00Z","timestamp":1754179200000},"content-version":"vor","delay-in-days":214,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scientifica"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Objective:<\/jats:bold>\n                    Hypervirulent\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (hvKp) is emerging and gaining notoriety due to the acquisition of drug resistance. Differentiation of hvKp from classical\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (cKp) is essential for prompt initiation of therapy to prevent metastasis, detection of occult abscesses, and site\u2010specific management for better patient outcomes.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    A total of 300\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates from various clinical specimens were collected from 256 patients to determine their clinical profiles, antibiograms, risk factors, and patient outcomes. Hypermucoviscosity was demonstrated via a phenotypic string test. The hvKp pathotype was classified by molecular detection of the virulence genes\n                    <jats:italic>rmpA<\/jats:italic>\n                    and\/or aerobactin\u2010\n                    <jats:italic>iucA<\/jats:italic>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    infections affected the older age group (&gt; 50\u2009years) of both sexes, with a male preponderance (62.89%). Urinary tract infections were the most common clinical presentation (37.33%). Among the 300 isolates, 17 (5.66%) possessed hypervirulence genes, and 281 (93.66%) isolates were string test positive. Pyogenic liver abscess was more frequently observed in hvKp infections (5.88%) than in cKp infections (1.76%). Multiple sites were involved in 35.29% of the hvKp infections. (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). Hypertension was the common comorbidity observed in the majority of the 256 patients (61.32%). The ICU stay (64.70%) predisposed patients to hvKp infections (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). Compared with hvKp, cKp presented high rates of resistance to antibiotics. Although extended\u2010spectrum beta\u2010lactamase (ESBL) producers were significantly more common in cKp, 41.1% of hvKp strains were ESBLs. Carbapenem resistance and multidrug resistance were observed in 35.29% of the hvKp strains. The mortality rate in patients infected with hvKp was 23.52%.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    The potential for occult abscess and metastasis with life\u2010threatening complications necessitates prompt, accurate identification of hvKp. Convergence of hvKp and cKp with shared traits poses a diagnostic and therapeutic dilemma for clinicians. The combination of genetic markers such as\n                    <jats:italic>rmpA<\/jats:italic>\n                    with\n                    <jats:italic>iucA<\/jats:italic>\n                    has high reported diagnostic accuracy. Further studies are needed to better characterize hvKp in the clinical laboratory.\n                  <\/jats:p>","DOI":"10.1155\/sci5\/8545710","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T05:34:12Z","timestamp":1754285652000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of Hypervirulence Genes and Drug Resistance in\n                    <i>Klebsiella pneumoniae<\/i>\n                    in Diagnostic Microbiology"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0610-1157","authenticated-orcid":false,"given":"Shamitha S.","family":"Rao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0374-975X","authenticated-orcid":false,"given":"Anisha M.","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,3]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-018-0302-9"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00001-19"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02867-23"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4498-x"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-022-00514-6"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4532707"},{"key":"e_1_2_11_7_2","volume-title":"Clinical Microbiology Procedure Handbook","author":"Isenberg H. 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