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Deep eutectic solvent was synthesized using ammonium acetate and lactic acid in different molar ratios and utilized as a sustainable extraction solvent under ultrasound\u2010assisted extraction conditions. Deep eutectic solvent\u2013based extraction was optimized to produce high amounts of bioactive compounds, and extracts obtained were compared with aqueous and methanolic solvents. Total phenolic content, antimicrobial activity against standard bacterial and fungal strains, as well as azole\u2010resistant and azole\u2010sensitive clinical isolates of\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      , were measured. Antioxidant capacity was calculated by 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl radical scavenging assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Deep eutectic solvent\u2013based extract was superior in extraction with greater total phenolic content (8.9\u2009\u00b1\u20090.4) and improved antimicrobial and antioxidant activity compared to the conventional extracts. Particularly, the deep eutectic solvent extract exhibited noteworthy inhibitory activity against Gram\u2010positive and Gram\u2010negative standard strains of bacteria, as well as standard and clinical yeast fungi. The biocompatibility of the deep eutectic solvent extract was assured by cytotoxicity analyses against 3T3 fibroblast cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These results demonstrate that DES\u2010based extraction serves as a green and effective method for isolating bioactive compounds from\n                      <jats:italic>Althaea officinalis<\/jats:italic>\n                      . This procedure deserves its potential use in the synthesis of novel antimicrobial agents.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/sci5\/9161837","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:41:59Z","timestamp":1772984519000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antimicrobial and Antioxidant Effects of Aqueous, Methanolic, and a Deep Eutectic Solvent\u2013Based Extracts of\n                    <i>Althaea officinalis<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"given":"Hossein","family":"Khodadadi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Ghasemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6135-9936","authenticated-orcid":false,"given":"Forough","family":"Karami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad","family":"Vaez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5703-5357","authenticated-orcid":false,"given":"Hasti","family":"Nouraei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimia","family":"Sahraeian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0314-7502","authenticated-orcid":false,"given":"Zahra","family":"Zareshahrabadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.121156"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtchem.2024.102069"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2025.107704"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2017.01.001"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125073"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/ijbm\/4251091"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/b210714g"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/d4nj05554c"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2017.02.014"},{"key":"e_1_2_14_10_2","first-page":"105","article-title":"Evaluation of the Antibacterial Activity of the Althaea officinalis L. 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