{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T09:48:56Z","timestamp":1775641736894,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":97,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["LH2024C061"],"award-info":[{"award-number":["LH2024C061"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFC2607402"],"award-info":[{"award-number":["2024YFC2607402"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    is an important emerging pathogen leading to serious clinical outcomes as the multidrug\u2010resistant (MDR) isolates arise. While\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    is a well\u2010known pathogen in human clinical settings, its risks in nonhuman hosts and potential for cross\u2010species transmission remain poorly understood. In this study, we performed whole\u2010genome sequencing on 112\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates from four host sources in Northeast China to elucidate their phylogenetic relationships and characterize their antimicrobial resistance (AMR) and virulence profiles. Our analysis identified 53 distinct sequence types (STs), 38 K\u2010locus (KL) types, and nine O\u2010loci. Notably, several strains with identical ST\u2013KL combinations were shared across different hosts: ST76\u2013KL5 was detected in both human and dairy cow sources; ST412\u2013KL57 was found in human and chicken; ST111\u2013KL63 was present in both pig and dairy cow sources, suggesting potential interspecies transmission. Antimicrobial susceptibility testing (AST) showed that nearly all human\u2010 and pig\u2010derived isolates were resistant to \u03b2\u2010lactams, quinolones, co\u2010trimoxazole, tetracyclines, and at least one aminoglycoside. In contrast, isolates from chickens and dairy cows exhibited lower resistance rates to these antibiotics. Overall, 88 of the 112 isolates (78.6%) were classified as MDR phenotype. Virulence gene screening indicated that 34 isolates carried the\n                    <jats:italic>iucABCD<\/jats:italic>\n                    \/\n                    <jats:italic>iutA<\/jats:italic>\n                    aerobactin synthesis cluster, a key marker for hypervirulence. These\n                    <jats:italic>iuc<\/jats:italic>\n                    \u2010positive strains fell into two distinct phylogenetic lineages, including\n                    <jats:italic>iuc1<\/jats:italic>\n                    and\n                    <jats:italic>iuc3<\/jats:italic>\n                    . It is necessary to conduct the regular monitoring of the antibiotic resistance and hypervirulence of clinical\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    from animals to prevent and control the transmission of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    along the food chain.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/1252580","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T08:55:03Z","timestamp":1775638503000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial Resistance, Virulence, and Genomic Characteristics of\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolated From Four Host Sources in Northeast China"],"prefix":"10.1155","volume":"2026","author":[{"given":"Qiu","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuilong","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaowei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofeng","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susu","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7390-6872","authenticated-orcid":false,"given":"Yao","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2041-2208","authenticated-orcid":false,"given":"Fang","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9171-8637","authenticated-orcid":false,"given":"Wanjiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,8]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani12223157"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2016.06.004"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-020-00875-w"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13787"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.105715"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.4137\/PMC.S14459"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17996-1"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000613"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2015.59"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.260"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.622891"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrp.2014.10.011"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S55778"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22718"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1030101"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.05.008"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab118"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00208-2"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.2002.0499"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.15931.1"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr507"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw359"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1239"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab301"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02128-23"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00001-19"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01750-20"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-018-0587-5"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-018-1475-3"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.04.004"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15721"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.858799"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01249"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-32337-8"},{"key":"e_1_2_11_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s43856-025-01112-1"},{"key":"e_1_2_11_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01537-18"},{"key":"e_1_2_11_4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