{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:07:17Z","timestamp":1785773237252,"version":"3.56.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":316,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1800304"],"award-info":[{"award-number":["2023YFD1800304"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20241859"],"award-info":[{"award-number":["BK20241859"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Pseudorabies virus (PRV) causes substantial economic losses in the global swine industry. Serological diagnosis plays a crucial role in its eradication. Here, we developed a competitive enzyme\u2010linked immunosorbent assay (cELISA) to detect antibodies against PRV glycoprotein D (gD). First, the recombinant gD ectodomain was expressed and purified to immunize mice, resulting in the generation of a monoclonal antibody (mAb 1D11) that targets gD. Subsequently, this antibody was conjugated with horseradish peroxidase (HRP), serving as the competing reagent. The cELISA was optimized under ideal conditions. Furthermore, validation using 204 swine serum samples\u2014comprising 110 PRV\u2010positive and 94 PRV\u2010negative samples\u2014demonstrated a high sensitivity and specificity, with a cutoff value of 46.16% inhibition determined by receiver operating characteristic (ROC) analysis (area under the curve\u2009=\u20090.995). Importantly, no cross\u2010reactivity was observed with antibodies against other tested swine viruses. Both intra\u2010 and inter\u2010assay coefficients of variation were found to be less than 10%, confirming high reproducibility of the assay results. When compared to a commercial PRV glycoprotein B (gB) ELISA kit (IDEXX), our cELISA exhibited strong agreement with\n                    <jats:italic>\u03ba<\/jats:italic>\n                    \u2009=\u20090.90. This robust, specific, and sensitive cELISA provides a reliable tool for large\u2010scale monitoring of PRV antibodies.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/1263531","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T10:13:16Z","timestamp":1763028796000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of a Competitive ELISA for Detecting Antibodies Against Pseudorabies Virus Glycoprotein D"],"prefix":"10.1155","volume":"2025","author":[{"given":"Lei","family":"Na","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ying","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Bo","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhe","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5192-2182","authenticated-orcid":false,"given":"Jinqiu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5690-2690","authenticated-orcid":false,"given":"Yan-Dong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-008-0278-4"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9070563"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2016.11.019"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1911.130177"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-020-00340-0"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.04.013"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-021-00347-1"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2018.1563459"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202407241"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2023.09.002"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201701129R"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8040090"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2016.09.004"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08269-3"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2157339"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14030572"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00944-21"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01821-24"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00636-25"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.24040"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1730245"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102511"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.139670"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01318-22"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2024.110164"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.133463"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2014.09.003"}],"container-title":["Transboundary 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