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To enhance diagnostic accuracy and epidemiological understanding of cetacean brucellosis in the Western Mediterranean Sea, we analyzed bacteriological, serological, and molecular data from 30 cetaceans belonging to three different species stranded along the coast of the Valencian Community (Spain) between 2011 and 2021.\n                    <jats:italic>Brucella ceti<\/jats:italic>\n                    infection was confirmed by bacteriological isolation in 14 animals (46.7%) and by genus\u2010specific qPCR in 15 cases (50%), with some discrepancies between methods. When feasible, serological analyses were performed using a commercial blocking ELISA (bELISA) and\/or the Rose Bengal agglutination test (RBT). In the absence of ELISA tests properly validated for its use in marine mammals, we assessed the optimum dilution and cut\u2010off of this ELISA kit using panels of gold\u2010standard sera from culture\u2010positive and brucellosis\u2010free dolphins. From a pathological perspective, 12 infected animals showed moderate to severe meningoencephalitis or meningoencephalomyelitis with lymphoplasmacytic infiltration. Additionally, whole\u2010genome sequencing (WGS) enabled the identification of two sequence types (STs), ST26 and ST49, indicating phylogenetic divergence. Our findings provide new insights into the phylogenetics of\n                    <jats:italic>B. ceti<\/jats:italic>\n                    and highlight the particular susceptibility of striped dolphins to this bacterium. The study also evidences the need for proper validation of the indirect diagnostic methods used for surveillance and seroepidemiological studies of brucellosis in marine mammals.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/2075116","type":"journal-article","created":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T08:38:59Z","timestamp":1769848739000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Towards Integrated Surveillance of Marine Brucellosis: Diagnostic and Phylogenetic Assessment of\n                    <i>Brucella ceti<\/i>\n                    in Stranded Dolphins of the Western Mediterranean 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