{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:40:13Z","timestamp":1792654813827,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":294,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>The influenza A virus (IAV) poses a significant threat to public health. Thus, antiviral drug research is a hotspot for IAV. All\u2010trans retinoic acid (ATRA), a bioactive metabolite of vitamin A, exhibits different effects on different subtypes of IAV. In this study, ATRA was found to inhibit canine influenza virus (CIV) replication in Madin\u2013Darby canine kidney (MDCK) and mink lung cells. Furthermore, the transcriptomic analysis revealed that ATRA significantly upregulated keratin 27 (KRT27), a cytoskeletal protein not previously associated with antiviral immunity. Overexpression of KRT27 suppressed CIV replication, while its knockdown enhanced viral load. Mechanistically, ATRA and KRT27 promoted autophagy, counteracting CIV\u2010mediated autophagy hijacking. Notably, ATRA\u2019s antiviral effect was species\u2010specific, showing no inhibition in human BEAS\u20102B or A549 cells. These findings identify ATRA\u2010induced KRT27 as a novel regulator of autophagy and innate immunity against CIV, offering a potential host\u2010directed therapeutic strategy against influenza viruses.<\/jats:p>","DOI":"10.1155\/tbed\/2088414","type":"journal-article","created":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:04:28Z","timestamp":1792652668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["All\u2010Trans Retinoic Acid Inhibits Canine Influenza Virus Replication Through Keratin 27\u2013Mediated Autophagy Modulation"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9924-5642","authenticated-orcid":false,"given":"Qi","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shanghai Academy of Agricultural Sciences  Shanghai 201106 China  saas.sh.cn"},{"name":"Shanghai Key Laboratory of Agricultural Genetic Breeding  Shanghai 201106 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8891-9756","authenticated-orcid":false,"given":"Shaotang","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine South China Agricultural University  Guangzhou 510642 China  scau.edu.cn"},{"name":"Department of Veterinary Medicine College of Animal Sciences Zhejiang University  Hangzhou 310058 China  zju.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine South China Agricultural University  Guangzhou 510642 China  scau.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benqiang","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shanghai Academy of Agricultural Sciences  Shanghai 201106 China  saas.sh.cn"},{"name":"Shanghai Key Laboratory of Agricultural Genetic Breeding  Shanghai 201106 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghua","family":"Cheng","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shanghai Academy of Agricultural Sciences  Shanghai 201106 China  saas.sh.cn"},{"name":"Shanghai Key Laboratory of Agricultural Genetic Breeding  Shanghai 201106 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Tao","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shanghai Academy of Agricultural Sciences  Shanghai 201106 China  saas.sh.cn"},{"name":"Shanghai Key Laboratory of Agricultural Genetic Breeding  Shanghai 201106 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shanghai Academy of Agricultural Sciences  Shanghai 201106 China  saas.sh.cn"},{"name":"Shanghai Key Laboratory of Agricultural Genetic Breeding  Shanghai 201106 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shanghai Academy of Agricultural Sciences  Shanghai 201106 China  saas.sh.cn"},{"name":"Shanghai Key Laboratory of Agricultural Genetic Breeding  Shanghai 201106 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine Shanghai Academy of Agricultural Sciences  Shanghai 201106 China  saas.sh.cn"},{"name":"Shanghai Key Laboratory of Agricultural Genetic Breeding  Shanghai 201106 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,22]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9100812"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268825000251"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01940-24"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-020-0287-5"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.03.021"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.09.009"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10030303"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2340"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-072610-145127"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022323"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2016.12.006"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-022-00869-y"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.15962"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01485-22"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2022.2067383"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00542-2"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41525-x"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14498"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202255192"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01757-16"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.01.006"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-025-01560-6"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-10-337"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-022-01809-y"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.456"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40246-021-00374-9"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.1897962"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.18014"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05792-11"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2021.198333"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2023.109824"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00964-2"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1580089"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009300"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1725375"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020105543"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14865"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/2088414","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/2088414","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/2088414","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:04:29Z","timestamp":1792652669000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/2088414"}},"subtitle":[],"editor":[{"given":"Nan-hua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tbed\/2088414"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/2088414","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2088414"}}