{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:12:00Z","timestamp":1789467120637,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":257,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32503051"],"award-info":[{"award-number":["32503051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2024J01332"],"award-info":[{"award-number":["2024J01332"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-41-18"],"award-info":[{"award-number":["CARS-41-18"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    (Pm) is an important veterinary and zoonotic pathogen that causes significant economic losses in poultry production. However, long\u2010term surveillance studies integrating antimicrobial resistance (AMR), biocide tolerance, and genomic epidemiology of Pm remain scarce. In this study, we investigated the antimicrobial susceptibility, biocide tolerance, and the phenotypic associations of 136 avian Pm isolates collected from six provinces in China between 2002 and 2024. Whole\u2010genome sequencing was performed to characterize population structure, identify antimicrobial resistance genes (ARGs), and assess genotype\u2013phenotype concordance. The A:L1:ST129 lineage remained the predominant clone throughout the 23\u2010year surveillance period, with a high prevalence of AMR\u2010associated traits observed within this lineage. Although resistance to several commonly used antimicrobial classes declined significantly after 2021, florfenicol resistance continued to increase, suggesting an emerging challenge for the clinical management of pasteurellosis. While the isolates generally exhibited low tolerance to the four representative biocides tested, phenotypic correlations were observed between AMR profiles and biocide tolerance patterns. Furthermore, substantial phenotype\u2013genotype discordance was observed, indicating that the presence of ARGs alone may not be sufficient to accurately predict antimicrobial susceptibility. Overall, this study provides a longitudinal assessment of long\u2010term AMR trends, biocide tolerance, and genomic epidemiology of avian Pm in China, offering epidemiological evidence for monitoring AMR trends and improving antimicrobial management strategies in poultry production.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/2248363","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:41:31Z","timestamp":1789461691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Twenty\u2010Three Years of Surveillance in Chinese Avian\n                    <i>Pasteurella multocida<\/i>\n                    Reveals Declining Antimicrobial Resistance but Increasing Therapeutic Challenges"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7457-2556","authenticated-orcid":false,"given":"Nansong","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2336-6681","authenticated-orcid":false,"given":"Weiwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qizhang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4005-8050","authenticated-orcid":false,"given":"Qiuling","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongchang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9579-9691","authenticated-orcid":false,"given":"Guanghua","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2011-5609","authenticated-orcid":false,"given":"Chunhe","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longfei","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wensong","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3654-8898","authenticated-orcid":false,"given":"Yu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00014-19"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2023.100593"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00473-0"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab462"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkad365"},{"key":"e_1_2_10_6_2","unstructured":"The Government of the People\u2019s Republic of China Notice of the Ministry of Agriculture and Rural Affairs on Issuing the \u201cNational Action Plan for Reducing the Use of Veterinary Antimicrobials (2021\u20132025)\u201c 2021 https:\/\/www.gov.cn\/zhengce\/zhengceku\/2021-10\/25\/content_5644815.htm."},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000001158"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-53391-2"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2017.10.005"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq112"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02428-24"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00188-20"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11111625"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/biology14081000"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-025-01950-2"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13122700"},{"key":"e_1_2_10_17_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; Clinical and Laboratory Standards Institute (CLSI)","author":"Institute C. L. S.","year":"2024"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13081724"},{"key":"e_1_2_10_19_2","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (EUCAST) Breakpoint Tables for Interpretation of MICs and Zone Diameters 2026 https:\/\/www.eucast.org\/ (accessed on 21 April 2026)."},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13051024"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.241455.118"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00483-19"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2022.109492"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2026.106524"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2024.110046"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2023.102830"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00651-25"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-59758-3"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwac269"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03132-23"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2025.140475"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00958-3"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0006-2017"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0022-2017"},{"key":"e_1_2_10_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9090614"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.117.1137"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176950"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2024.120075"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.animal.2020.100123"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(10)70053-0"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00125-13"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/2248363","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/2248363","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/2248363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:41:34Z","timestamp":1789461694000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/2248363"}},"subtitle":[],"editor":[{"given":"Zhong","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tbed\/2248363"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/2248363","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2248363"}}