{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:42:16Z","timestamp":1773009736252,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":348,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1800504"],"award-info":[{"award-number":["2023YFD1800504"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    The red\u2010crowned crane (\n                    <jats:italic>Grus japonensis<\/jats:italic>\n                    ) is one of the rarest cranes with a global population of less than 4000 individuals. The population of red\u2010crowned crane could be influenced by health threats, including metabolic and infectious diseases. In the Wildlife Rescue Center of Suining County of Jiangsu Province, gouty arthritis (GA) was observed in all four red\u2010crowned cranes since March 2024. A pooled fecal supernatant was first submitted to metagenomics sequencing for screening disease\u2010associated pathogens. Enterobacteria phage phiEcoM\u2010GJ1 was detected as the predominant virus while\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Aeromonas hydrophila<\/jats:italic>\n                    were the dominated bacteria in the mixed fecal sample from red\u2010crowned cranes. The 16S rRNA gene sequencing was further performed on both the mixed fecal sample and four individual samples, which showed that\n                    <jats:italic>Escherichia-Shigella<\/jats:italic>\n                    ,\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    , and\n                    <jats:italic>Enterococcus<\/jats:italic>\n                    were the most abundant gut flora in both mixed and individual fecal samples. Furthermore, bacteria isolation and identification with matrix\u2010assisted laser desorption ionization\u2010time of flight mass spectrometry (MALDI\u2010TOF\/MS) confirmed that\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    was predominant (19\/29 colonies, 65.52%) in the feces. Therefore, anti\u2010uricacid and antibacteria treatments using plantain herb, doxycycline, Vitamin AD3 and multivitamin B were adopted, leading to a full behavioral recovery within 1\u2009month. Overall, this case\u2010based observational study provides first clue on the gut\u2010joint axis in red\u2010crowned cranes, supporting that gut microbiota dysbiosis is closely associated with GA in red\u2010crowned cranes.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/2422900","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T09:04:21Z","timestamp":1765789461000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Throughput Sequencings Revealed That Gut Microbiota Dysbiosis is Implicated in Gouty Arthritis of Red\u2010Crowned Crane (\n                    <i>Grus japonensis<\/i>\n                    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