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In 2018, the virus acquired four genes (\n                    <jats:italic>M157L<\/jats:italic>\n                    ,\n                    <jats:italic>M158L<\/jats:italic>\n                    ,\n                    <jats:italic>M159L<\/jats:italic>\n                    and\n                    <jats:italic>M160L<\/jats:italic>\n                    ) from a yet unidentified source and gained the capacity to infect the Iberian hare (\n                    <jats:italic>Lepus granatensis<\/jats:italic>\n                    ). The new viral strain, termed ha\u2010MYXV (hare myxoma virus), continues to circulate within Iberian hare populations, causing a dramatic decline in numbers of this species. To analyse the genetic stability of the emergent ha\u2010MYXV, following its host species jump, we sequenced the novel four\u2010gene cassette in 106 samples collected over an 8\u2010year period (2018\u20132025) from Iberian hares and wild and domestic rabbits throughout Spain. Samples were either collected as part of an active surveillance campaign or through passive sampling of diseased animals. Our results show that M157 and M158 are completely conserved in all samples, while M160 demonstrated occasional sporadic mutations. However, gene\n                    <jats:italic>M159L<\/jats:italic>\n                    , the C7L\u2010like host range virulence factor, demonstrated simple sequence repeat (SSR) trinucleotide expansions and contractions in ~60% of samples analysed. Further analysis showed that ha\u2010MYXV contains unique SSR in genes associated with early stop mutations. Sequencing of the ha\u2010MYXV\u2010specific SSR regions in field samples shows a large degree of variation. These findings indicate that the variable nature of SSR in the emerging virus contrasts with the stability of the other novel genes present in the ha\u2010MYXV genome. This study highlights the need for continued surveillance in order to better understand virus evolution following a cross\u2010species virus transmission event.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/3847131","type":"journal-article","created":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T12:02:38Z","timestamp":1776513758000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enrichment and Reduction of Microsatellite Regions in the Myxoma Virus Genome Following Species Jump to the Iberian Hare (\n                    <i>Lepus granatensis<\/i>\n                    )"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6360-3755","authenticated-orcid":false,"given":"A.","family":"Men\u00e9ndez-Manj\u00f3n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8267-0953","authenticated-orcid":false,"given":"B.","family":"Cardoso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Castro-Scholten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ag\u00fcero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Duran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Buitrago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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