{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T19:57:37Z","timestamp":1784231857954,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":327,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1800500"],"award-info":[{"award-number":["2023YFD1800500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Porcine deltacoronavirus (PDCoV) is an emerging enteric pathogen that causes substantial economic losses in the global swine industry. Although neutralizing antibodies (NAbs) are a key indicator of vaccine efficacy, their diagnostic concordance with IgG\/IgA levels measured by indirect enzyme\u2010linked immunosorbent assays (iELISAs) targeting major structural proteins has not been systematically evaluated. In this study, the full\u2010length spike (S), S1 domain, and receptor\u2010binding domains (RBD) from a highly virulent PDCoV strain were expressed in CHO cells. At the same time, the membrane (M) and nucleocapsid (N) proteins were produced in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (\n                    <jats:italic>E. coli<\/jats:italic>\n                    ). Following initial reactivity screening via protein microarray, S, S1, RBD, and N were chosen to establish iELISAs for detecting IgG and IgA antibodies in serum and milk samples. Evaluating iELISAs\u2019 specificity revealed cross\u2010reactivity of anti\u2010S IgA and anti\u2010N IgA with porcine epidemic diarrhea virus (PEDV) antibody\u2010positive sera. Analysis of 75 clinical pig serum samples, and 75 colostrum samples demonstrated that IgA\u2010based iELISAs had superior diagnostic concordance with virus neutralization test (VNT) results than IgG\u2010based iELISAs, with the S1\u2010IgA iELISA showing the highest concordance (93.3%). Furthermore, IgA antibody levels correlated more strongly with neutralizing titers (NTs) than IgG. These findings validate the S1\u2010IgA iELISA as a robust, high\u2010throughput serological tool for assessing PDCoV immunity in pigs.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/3988285","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T10:09:57Z","timestamp":1763978997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development and Comparison of Indirect ELISAs for Detecting IgG and IgA Antibodies Against Major Structural Proteins of Porcine Deltacoronavirus With Virus Neutralization as a 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