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In China, the cocirculation of diverse strains drives ongoing viral evolution and the emergence of novel recombinants, posing serious challenges for disease control. Here, we report the isolation and characterization of a novel PRRSV strain (JS\u2010YZ\/2023) from a PRRS\u2010suspected pig farm. Genomic analysis revealed a unique recombinant pattern between NADC30\u2010like and HP\u2010PRRSV lineages, distinct from previously reported recombinants. Experimental infection in piglets demonstrated significant pathogenicity, with persistent fever, severe anorexia, progressive weight loss, and pathological lesions including interstitial pneumonia with lymphocyte infiltration and marked disruption of intestinal villi. Notably, JS\u2010YZ\/2023 exhibited strong intestinal tropism, inducing a local cytokine storm in the small intestine and showing PRRSV\u2010N protein antigen accumulation in crypt epithelial cells, as detected by immunohistochemistry. In addition, we successfully constructed a full\u2010length infectious cDNA clone of JS\u2010YZ\/2023 incorporating a green fluorescent protein (GFP) reporter gene (rGFP\u2010JS\u2010YZ\/2023). This engineered virus provides a valuable tool for investigating the pathogenic mechanisms of NADC30\u2010like recombinants and facilitating vaccine development.<\/jats:p>","DOI":"10.1155\/tbed\/4893251","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T11:32:09Z","timestamp":1766748729000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Characterization and Pathogenicity of a Novel Recombinant NADC30\u2010Like PRRSV Strain Isolated From Piglets Inducing Intestinal Inflammation, and Construction of Its Infectious 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