{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:50:38Z","timestamp":1791532238314,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":281,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20363"],"award-info":[{"award-number":["U24A20363"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Feline calicivirus (FCV) is a common pathogen causing upper respiratory tract disease in domestic cats. Nonetheless, its threat and genetic features in endangered wild felids remain poorly understood. In this study, a novel FCV strain from a captive South China tiger (\n                    <jats:italic>Panthera tigris amoyensis<\/jats:italic>\n                    ) was isolated, designated as FCV LYH1224. Viral RNA was detected in multiple tissues of the deceased tiger, including the heart, liver, spleen, lungs, and kidneys, suggesting broad tissue tropism and FCV contributed substantially to the fatal outcome. In vitro growth kinetics showed that the virus replicates efficiently in Crandell\u2010Rees feline kidney (CRFK) cells. Whole\u2010genome sequencing and phylogenetic analysis indicated that it was classified as genotype II (GII) and exhibited unique evolutionary lineages. Within GII, it clusters closely with the previously reported tiger\u2010derived strain FCV\u2010YH\/16 but exhibits an independent branch in the VP1 gene tree, suggesting complex genetic variation among FCV strains from diverse wildlife origins. Sequence identity analysis indicated that FCV LYH1224 was genetically distant from vaccine strains F9 and 2280, with whole\u2010genome nucleotide identity of 76.6% and 77.0%, respectively. Notably, the VP1\u2010E region displayed the lowest identity, with nucleotide identity of 68.1% and 66.7%, respectively. Analysis of key amino acid residues revealed distinct substitutions within the neutralizing epitope (aa 445\u2013451) and common mutations at virulence\u2010related positions 452A and 492I, which were absent in reference strains, suggesting potential antigenic diversity and host\u2010specific adaptations. This study provides a comprehensive genomic characterization of FCV strain in South China tigers, highlighting the genetic diversity and distinctive antigenic properties observed in this isolate compared with existing strains. The considerable genetic divergence from vaccine strains emphasizes the importance of ongoing surveillance and the inclusion of wildlife\u2010derived strains in vaccine development strategies.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/4900253","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:22:23Z","timestamp":1791530543000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Diversity and Biological Characteristics of a Novel Feline Calicivirus Strain Isolated From Zoo\u2010Captive South China Tiger (\n                    <i>Panthera tigris amoyensis<\/i>\n                    )"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5329-0359","authenticated-orcid":false,"given":"YuShan","family":"Ding","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine South China Agricultural University  Guangzhou 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