{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T09:14:44Z","timestamp":1783674884826,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":190,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Balantioides coli<\/jats:italic>\n                    is the only ciliate with significant implications for both human and animal health. Regarding animals,\n                    <jats:italic>B. coli<\/jats:italic>\n                    primarily infects pigs. However, there is a lack of data regarding\n                    <jats:italic>B. coli<\/jats:italic>\n                    in pigs in Shanxi, a province in northern China covering 156,700\u2009km\n                    <jats:sup>2<\/jats:sup>\n                    where pig farming is a major agricultural industry. In this study, 341 pig fecal samples collected from this province were examined for the presence of\n                    <jats:italic>B. coli<\/jats:italic>\n                    using a traditional polymerase chain reaction (PCR) method targeting the \u03b2\u2010tubulin gene. The results showed that the overall prevalence of\n                    <jats:italic>B. coli<\/jats:italic>\n                    in the sampled pigs was 75.1%, and its prevalence varied significantly across different regions. Among the polymorphic sequences identified in this study, the genotype with zoonotic potential (genotype I) was observed. In addition, we established a cross\u2010priming amplification (CPA) strategy\u2013assisted lateral flow immunoassay (LFIA) biosensor for the detection of\n                    <jats:italic>B. coli<\/jats:italic>\n                    . The whole process from amplification to visual readout can be completed within 63\u2009min without any special instrument. The biosensor exhibited a sensitivity of 100\u2009copies\/\u00b5L of plasmid DNA and specifically recognized\n                    <jats:italic>B. coli<\/jats:italic>\n                    . The results of the diagnostic performance analysis revealed that the biosensor accurately distinguished between PCR\u2010positive and \u2010negative fecal samples. Our results contribute to expanding our understanding of the geographical distribution and genetic diversity of\n                    <jats:italic>B. coli<\/jats:italic>\n                    in pigs and have important implications for controlling\n                    <jats:italic>B. coli<\/jats:italic>\n                    infection in pigs and humans. Also, this study provides a novel molecular tool for the detection of\n                    <jats:italic>B. coli<\/jats:italic>\n                    , particularly in resource\u2010limited settings.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/4982345","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T08:19:27Z","timestamp":1783671567000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection of\n                    <i>Balantioides coli<\/i>\n                    in Pigs Employing the Traditional PCR and a Novel Cross\u2010Priming Amplification\u2010Based Lateral Flow 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