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In this study, a novel recombinase\u2010aided amplification (RAA) combined with lateral flow dipstick (LFD) assay was developed and validated for the rapid and specific detection of\n                    <jats:italic>S. papillosus<\/jats:italic>\n                    infection in goats and sheep, targeting the 18S ribosomal RNA (18S rRNA) gene. A total of 815 fecal samples were collected from nine provinces in China, encompassing major goats and sheep production regions. The RAA\u2010LFD assay exhibited high sensitivity, with a minimum detection limit of 15 eggs per gram (EPG) of feces. Clinical fecal examination yielded a positive rate of 62.30% (433\/695), while the RAA\u2010LFD assay achieved a positive rate of 58.28% (475\/815), indicating that the diagnostic accuracy of the RAA\u2010LFD assay is consistent with that of fecal examination. Compared with conventional fecal examination, the RAA\u2010LFD assay offers superior rapidity, portability, and sensitivity, presenting a valuable diagnostic tool for large scale epidemiological surveillance and point\u2010of\u2010care applications, particularly in resource constrained environments.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5004834","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T09:44:04Z","timestamp":1761385444000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Survey of\n                    <i>Strongyloides papillosus<\/i>\n                    Infection in Goats and Sheep in Part of China Using RAA\u2010LFD 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