{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:37:59Z","timestamp":1787593079891,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":352,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["TD2022C006"],"award-info":[{"award-number":["TD2022C006"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>Enzootic nasal tumor virus (ENTV) is the etiological agent responsible for enzootic nasal adenocarcinoma (ENA), a chronic and contagious disease predominantly affecting sheep and goats. ENTV is classified into two distinct types: ENTV\u20101, which infects sheep, and ENTV\u20102, which infects goats. ENA has been globally reported in small ruminant\u2010rearing regions, causing significant mortality and substantial economic impacts in affected flocks. There is currently no standardized detection method for ENA. In this study, we successfully generated a monoclonal antibody (mAb) and a polyclonal antibody (pAb) against the ENTV\u20102 capsid protein (p27), and identified the epitope of the mAb, which was found to be highly conserved among different ENTV\u20102 isolates. An antigen capture ELISA (acELISA) was then successfully developed using the mAb as the capture antibody and the pAb as the detection antibody to specifically detect p27 of ENTV\u20102 in nasal secretions. The cut\u2010off value of the acELISA was determined to be 0.1052 by analyzing S\/P values. The detection limit of this assay was 0.16\u2009ng\/mL of rp27 protein and equivalent to 844\u2009copies\/\u03bcL of ENTV\u20102 RNA. Specificity tests showed that the method had no cross\u2010reaction with other prevalent small ruminant pathogens. The coincidence rates of the developed acELISA compared with western blotting and qRT\u2010PCR assays were 98.95% (189\/191) and 96.34% (184\/191), respectively. Furthermore, the acELISA was applied to assess ENTV\u20102 in 1228 clinical nasal swab samples collected from seven provinces in China. The results demonstrated that the positivity rate varied between 0.00% and 28.21%. In conclusion, we successfully developed an acELISA with high specificity, sensitivity and reproducibility for the detection of ENTV\u20102 antigen. This high\u2010throughput method for the detection of ENTV\u20102 represents a significant advancement in the field and may contribute to the prevention and control of ENTV\u20102.<\/jats:p>","DOI":"10.1155\/tbed\/5514208","type":"journal-article","created":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T06:53:26Z","timestamp":1766213606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development and Application of an Antigen Capture ELISA for the Detection of Enzootic Nasal Tumor Virus\u20102"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0079-5348","authenticated-orcid":false,"given":"Yang","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8127-4796","authenticated-orcid":false,"given":"Jinling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0771-1791","authenticated-orcid":false,"given":"Jiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qixin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingwu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunze","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kui","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2195-6813","authenticated-orcid":false,"given":"Weiguo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2963-0364","authenticated-orcid":false,"given":"Xiaohua","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5564-2487","authenticated-orcid":false,"given":"Xue-Feng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4521-4099","authenticated-orcid":false,"given":"Xiaojun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-55638-8_8"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-44-66"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-019-04211-2"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/v11100956"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.5.3986-3993.1999"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2009.12.013"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000001982"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-023-12504-5"},{"key":"e_1_2_14_9_2","unstructured":"WOAH Ovine Pulmonary Adenocarcinoma (Adenomatosis) WOAH Terrestrial Manual-Chapter 3.8.82021."},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2010.04.002"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.064956-0"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.810977"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/10406387241310204"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci11060248"},{"key":"e_1_2_14_15_2","unstructured":"FuS. 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