{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:26:16Z","timestamp":1775060776995,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":55,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["408696\/2024-9"],"award-info":[{"award-number":["408696\/2024-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Accurate diagnosis of bovine tuberculosis (bTB) remains a critical challenge for disease control and eradication programs. While cell\u2010mediated immune (CMI) response tests such as the comparative cervical intradermal tuberculin test (CCITT) are widely used, their sensitivity is limited, especially in later stages of infection. Antibody\u2010based assays, such as enzyme\u2010linked immunosorbent assays (ELISAs), may offer complementary detection and improve case identification. This study aimed to comparatively evaluate two indirect ELISA tests for the detection of antibodies against\n                    <jats:italic>Mycobacterium bovis<\/jats:italic>\n                    in naturally infected cattle from Southern Brazil. One ELISA test is in an experimental phase and uses a chimeric antigen comprising fragments of MPB70, MPB83, and ESAT\u20106 proteins. The second is a commercially available ELISA registered with the WOAH, based on MPB70 and MPB83 antigens. Serum samples were collected from 147 cattle across nine herds with known bTB epidemiological histories. All animals underwent CCITT, and a subset was subjected to post\u2010mortem examination, culture, and nested\u2010PCR. The experimental ELISA identified 16.6% of animals as seropositive, while the commercial ELISA identified 13.0%, both exceeding the 4.4% apparent prevalence detected by CCITT. Both ELISAs showed low sensitivity (&lt;30%) when compared to culture or PCR\u2010confirmed cases but were able to detect additional infected animals missed by CCITT. These findings support the use of serological tests as complementary tools to enhance bTB detection in cattle and inform surveillance and eradication strategies in endemic regions.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5527662","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:38:54Z","timestamp":1772991534000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Evaluation of ELISA Tests for Bovine Tuberculosis Detection: Implications for Improved Disease Control"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0338-1741","authenticated-orcid":false,"given":"Carlos Eug\u00eanio","family":"S. Vidal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1169-8196","authenticated-orcid":false,"given":"Ingrid Ieda","family":"F. Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3084-172X","authenticated-orcid":false,"given":"Carlos Alberto","family":"N. Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7069-8035","authenticated-orcid":false,"given":"J\u00e9ssica","family":"F. Camargo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6351-3799","authenticated-orcid":false,"given":"Cristina","family":"P. Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9149-5185","authenticated-orcid":false,"given":"Ana Luiza","family":"A. R. Os\u00f3rio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1379-8344","authenticated-orcid":false,"given":"Denis","family":"A. Spricigo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2034-3655","authenticated-orcid":false,"given":"Felipe","family":"Libardoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2334-5904","authenticated-orcid":false,"given":"Fl\u00e1bio","family":"R. Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9309-4717","authenticated-orcid":false,"given":"Cynthia","family":"Mantovani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9637-4028","authenticated-orcid":false,"given":"Agueda","family":"C. Vargas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30139-6"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2023.100495"},{"key":"e_1_2_10_3_2","unstructured":"Ministry of AgricultureandLivestock and Food Supply (MAPA) Instru\u00e7\u00e3o Normativa SDA N\u00b0 10 de 3 de Mar\u00e7o de 2017 2017 Aprova o Regulamento t\u00e9cnico Do Programa Nacional De Controle e Erradica\u00e7\u00e3o DA Brucelose e DA Tuberculose Animal (PNCEBT)."},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711403410"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.3.571-578.2001"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5344-5349.1998"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00061-06"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2009.02256.x"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.11.032"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1865-1682.2010.01148.x"},{"key":"e_1_2_10_11_2","unstructured":"WOAH IDEXXM. bovisAntibody Test kit\u00ae OIE Procedure for Registration of Diagnostic Kits: Abstract Sheet MBAT Kit 2012 WOAH."},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352009000700012"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162459"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1590\/s0100-736x2017001200004"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/2193-1801-1-77"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.08.008"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.21.1.38"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-9-2131"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091023"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822014000200035"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5877(92)90082-Q"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00420-10"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00119-15"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2013.02.016"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5527662","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/5527662","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5527662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T14:47:56Z","timestamp":1775054876000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/5527662"}},"subtitle":[],"editor":[{"given":"Yong-guo","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tbed\/5527662"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/5527662","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2024-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5527662"}}