{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:26:54Z","timestamp":1775060814145,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":33,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1800500"],"award-info":[{"award-number":["2023YFD1800500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Senecavirus A (SVA) is an emerging swine pathogen that causes vesicular disease, which presents clinically indistinguishable signs from other vesicular diseases. To enable differentiation of infected from vaccinated animals (DIVA), we developed a novel protein microarray for dual serological detection of antibodies against SVA structural (VP2\u2010VP3\u2010VP1) and non\u2010structural (3AB\u20103C) proteins. The assay was based on two novel His\u2010tagged tandem antigens, designed from immunodominant B\u2010cell epitopes, which were expressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (\n                    <jats:italic>E. coli<\/jats:italic>\n                    ), purified, and spotted onto a poly(dimethylsiloxane) (PDMS) substrate. Results were quantified by spot gray values to calculate sample\u2010to\u2010positive (S\/P) ratios, with cut\u2010off values set at S\/P \u22650.651 for VP2\u2010VP3\u2010VP1 and S\/P \u22650.607 for 3AB\u20103C. The microarray successfully differentiated inactivated\u2010vaccine\u2010immunized animals (positive only for VP2\u2010VP3\u2010VP1) from live SVA\u2010challenged animals (positive for both antigens). In live SVA\u2010challenged pigs, seroconversion to the structural protein antigen VP2\u2010VP3\u2010VP1 occurred 4 days earlier than the non\u2010structural protein antigen 3AB\u20103C, identifying it as a sensitive early diagnostic marker. Clinical validation demonstrated 97.5% concordance with the virus neutralization test (VNT), confirming the microarray as a reliable, high\u2010throughput tool for DIVA serological testing.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5543555","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T17:18:18Z","timestamp":1770139098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and Application of a Novel Protein Microarray for Serological Detection and Differentiation of Senecavirus 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