{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:04:21Z","timestamp":1789538661761,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":257,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD1800100"],"award-info":[{"award-number":["2024YFD1800100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32473058"],"award-info":[{"award-number":["32473058"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Babesia gibsoni<\/jats:italic>\n                    is a globally distributed tick\u2010borne apicomplexan parasite that threatens dog health, yet its transmission dynamics in\n                    <jats:italic>Haemaphysalis longicornis<\/jats:italic>\n                    remain incompletely defined. Under controlled laboratory conditions, a\n                    <jats:italic>Babesia<\/jats:italic>\n                    \u2010free parthenogenetic\n                    <jats:italic>H. longicornis<\/jats:italic>\n                    colony was used to evaluate transovarial transmission, transmission competence of the resulting F1 stages, retention of transmission competence after feeding on non\u2010susceptible rabbits, and post\u2010molt transmission after larval or nymphal acquisition feeding. Tick samples from the corresponding experimental lineages were screened by pooled internal transcribed spacer (ITS)\u2010targeted nested PCR at the egg, larval, nymphal, and adult stages. Transmission competence was evaluated by infesting splenectomized recipient dogs with the remaining ticks from the corresponding PCR\u2010screened lineages, followed by PCR and blood\u2010smear monitoring. Ticks from a PCR\u2010positive transovarial F1 lineage transmitted\n                    <jats:italic>B. gibsoni<\/jats:italic>\n                    to recipient dogs at the larval, nymphal, and adult stages. Within the transovarial F1 lineage, transmission competence was retained after one or two developmental\u2010stage feedings on non\u2010susceptible rabbits. In contrast, no evidence of post\u2010molt transmission to recipient dogs was observed after larval or nymphal acquisition feeding. Collectively, these findings provide laboratory evidence for transovarial transmission of\n                    <jats:italic>B. gibsoni<\/jats:italic>\n                    and transmission competence of the resulting F1 larval, nymphal, and adult stages in the tested parthenogenetic\n                    <jats:italic>H. longicornis<\/jats:italic>\n                    colony.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5585506","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:29:43Z","timestamp":1789536583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Laboratory Evidence for Transovarial Transmission of\n                    <i>Babesia gibsoni<\/i>\n                    by\n                    <i>Haemaphysalis longicornis<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4367-886X","authenticated-orcid":false,"given":"Yaxin","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangwei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0750-4455","authenticated-orcid":false,"given":"Muxiao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2407-2768","authenticated-orcid":false,"given":"Fen","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5162-4495","authenticated-orcid":false,"given":"Junlong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5453-4425","authenticated-orcid":false,"given":"Lan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1596-0"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-2-S1-S4"},{"key":"e_1_2_11_3_2","first-page":"163","article-title":"Canine Babesiosis in China Caused by Babesia Gibsoni: A Molecular Approach","volume":"9","author":"Yao D. W.","year":"2014","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01577"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.01.011"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2022.03.001"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04232-w"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2722-y"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.09.022"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(72)90057-4"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjy072"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2542-5196(20)30145-5"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00429"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2015.1035769"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.12-0175"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.2307\/3276992"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjab015"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crpvbd.2024.100199"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020092"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.01.035"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2009020"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00248"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14822"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.12.001"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1259-1263.2002"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2025055"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12020114"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12040823"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-09153-x"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5585506","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/5585506","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5585506","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:29:44Z","timestamp":1789536584000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/5585506"}},"subtitle":[],"editor":[{"given":"Ruofeng","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tbed\/5585506"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/5585506","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-03-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5585506"}}